Avaliação do antígeno rKDDR-Plus para o diagnóstico da Leishmaniose Visceral Canina em abrigos de animais de Aracaju/SE

Detalhes bibliográficos
Ano de defesa: 2020
Autor(a) principal: Gomes, Ana Carolina Amado
Orientador(a): Fujiwara, Ricardo Toshio
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Pós-Graduação em Biologia Parasitária
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://ri.ufs.br/jspui/handle/riufs/15714
Resumo: Visceral Leishmaniasis (VL) is a neglected tropical disease that can lead to death if not properly diagnosed and treated. The domestic dog is considered the most important reservoir due to its proximity to man. Correct reservoir diagnosis is important to control disease transmission. In order to improve or diagnose the LV canine, a recombinant antigen was developed consisting of 15.3 repeated motifs and 39 kinin amino acids called rKDDR-Plus. The aim of this study was to evaluate the rKDDR-Plus antigen for the diagnosis of Canine Visceral Leishmaniasis in dogs kept in sheltersin Aracaju / SE. As a gold standard, a PCR for Leishmania sp. The rKDDRPlus immunochromatographic test showed greater sensitivity (57%); compared to DPP BioManguinhos® (45%). Both testicles have the same specificity (69%). The rKDDR-Plus was more accurate in dogs diagnosed with VL in endemic areas when it heard a rapid test and currently uses the screening ELISA rKDDR is more sensitive, 49% with 95% CI: 40.71-57.78; in the ELISA rKDDR-Plus, it showed greater specificity, 81% with 95% CI: 62.12-91.49. When compared to the other two tests, rKDDR-Plus showed the largest area of the ROC curve, followed by Gross Extract and rKDDR. Conclude that rKDDR-Plus can be used as a tool to diagnose a CVL.