Efeito da fotobiomodulação a laser de λ808 nm e λ660 nm no processo de reparo periodontal de dentes reimplantados em ratos

• Ano de defesa: 2016
• Autor(a) principal: Matos, Felipe de Souza
• Orientador(a): Ribeiro, Maria Amália Gonzaga
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Odontologia
• País: Brasil
• Palavras-chave em Português: Avulsão dentária; Reimplante dentário; Lasers; Dentes – Raízes – Reabsorção; Tooth replantation
• Palavras-chave em Inglês: Tooth avulsion
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://ri.ufs.br/handle/riufs/5896

The tooth replantation is considered the ideal treatment in cases of dentoalveolar trauma with avulsion. However, even respecting the optimal conditions of extra-alveolar time or storage, much of the replanted teeth is lost mainly by the occurrence of external root resorption. Thus, this study evaluated through histomorphometric analysis the effect of laser photobiomodulation (LPBM) at λ808 nm and λ660 nm and of storage media on the periodontal repair process of replanted teeth in rats. The research was conducted on the in vivo experimental study design, randomized and with simple blinding. Maxillary right incisors were extracted from sixty Wistar rats and randomly assigned to six groups (n = 10): PN, WM and SM, in which the teeth were stored in paper napkin, whole milk and soy milk, respectively, for 45 minutes; and PNL, WML and SML, in which the teeth were stored in the same storage media, but the root surface and the alveolus were treated with LPBM at λ808 nm before the replantation (GaAlAs, CW, 100 mW, 61 J/cm2) and the buccal and palatal mucosa of the alveolus with LPBM at λ660 nm after the replantation (InGaAlP, CW, 100 mW, 61 J/cm2). Five rats from each group were euthanized after 15 or 30 days. The right hemimaxilla including the replanted incisor was removed and processed for histomorphometric analysis of inflammatory root resorption areas, replacement root resorption areas, perimeter with ankylosis and periodontal repair, and areas of type I and III collagen deposition, using the ImageJ 1.50b software. Data were analyzed statistically by the ANOVA test followed by Tukey's multiple comparison test (α = 5%). All data presented normal distribution according to the Shapiro-Wilk test. WM and SM showed smaller root resorption areas and higher collagen deposition in both experimental periods and SM the greatest perimeter of repair. LPBM reduced the occurrence of root resorption only in PNL group, both in 15 and in 30 days, and increased the perimeter of periodontal repair in all groups at 30 days. There was higher collagen deposition in the irradiated groups independent of the experimental period. It was concluded that the LPBM protocol at λ808 nm and λ660 nm, as well as whole milk and soy milk favored the periodontal repair process of replanted teeth in rats.