Qualidade fisiológica de sementes de cultivares de alface sob diferentes temperaturas na germinação

• Ano de defesa: 2016
• Autor(a) principal: Almeida, Fernando Araujo de
• Orientador(a): Silva-Mann, Renata
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Agricultura e Biodiversidade
• País: Brasil
• Palavras-chave em Português: Alface; Germinação; Fisiologia vegetal; Isoenzimas; Lactuca sativa L.; Termotolerância
• Palavras-chave em Inglês: Isoenzymes; Thermotolerance
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::AGRONOMIA
• Link de acesso: https://ri.ufs.br/handle/riufs/3028

The objective of this study was to evaluate the physiology and biochemistry of lettuce seed through germination and expression of the enzymes alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), catalase (CAT), Esterase (EST), pyruvate decarboxylase (PDC) and glutamate oxalacetate transferase (GOT) at different temperatures. Seeds of four lettuce cultivares were submitted to germination and the first germination was tested under the temperatures of 20, 25, 30, 35, 40 and 42°C. The germination speed index (IVG), the percentage of germination were estimated, and the expression of the enzyme alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), catalase (CAT), esterase (EST), pyruvate decarboxylase (PDC) and glutamate oxalacetate transferase (GOT) evaluated. The experimental design was completely randomized, in a 4x6 factorial scheme, with four cultivars and six different temperatures, with four replications. The highest values of germination and vigor were observed for cv. Everglades at 35ºC, proving the thermotolerance. The catalase may be considered a marker for identification of thermotolerant lettuce cultivars. Cultivar Everglades has potential for use in lettuce breeding programs aiming at temperature tolerance during germination.