Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA

• Ano de defesa: 2019
• Autor(a) principal: Prado, Vilma Menezes de Jesus
• Orientador(a): Moraes, Valéria Regina de Souza
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Programa de Pós-Graduação: Pós-Graduação em Química
• Palavras-chave em Português: Plantas medicinais; Plantas da caatinga; Euphorbiaceae; Cromatogramas; Agentes antineoplásticos; Croton grewioides Baill.; Cromatogramas fingerprint; Flavonoides; Atividade antitumoral; Atividade anticolinesterásica; Inibidores de colinesterase
• Palavras-chave em Inglês: Fingerprint chromatograms; Flavonoids; Antitumoral activity; Anticholinesterasic activity
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: http://ri.ufs.br/jspui/handle/riufs/10994

The species Croton grewioides Baill., popularly known as "canelinha" or "canelinha-de-odor", has been used for the treatment of influenza, cough, fever and headache, however the study of its phytochemical composition is limited. A method by liquid chromatography for the differentiation of leaf extracts (hydroalcoholic, aqueous and methanolic) of four accessions of C. grewioides from two collections (winter and summer) has been developed. Some chemical constituents from aqueous extract of accession 107 (summer collection) were isolated by semi-preparative LC, in addition to the evaluation of in vitro activities against the tumor cell lines HepG2 (human hepatocellular carcinoma) and HL-60 (human leukemia) and anticholinesterase of the extracts and the partitions from methanolic extract.Thus, the fingerprint chromatograms of all extracts were obtained for the first time, which could be compared with the aid of chemometric tools. The results showed that only the chemical profile of the methanolic extracts of accessions 101 and 113 were influenced by the seasonality. The structures of three isolated metabolites were identified by 1H and 13C NMR (uni and bidimensional), namely: quercetin-3-O- [α-Rhamnopyranosyl- (1-2) -α- Rhamnopyranosyl- (1-6) -β-glucopyranoside], quercetin-3-O-glucoside and quercetin-3-O-methyl. Four hexanic partitions were considered active against the HL-60 cell, exhibiting inhibition values of 83.75; 84.43; 84.66 and 92.93%.