Efeito agudo do RAST TEST sobre o estresse oxidativo e os marcadores indiretos de dano muscular em corredores adolescentes

• Ano de defesa: 2015
• Autor(a) principal: Santos, Patrícia Morgana Ferreira
• Orientador(a): Pardono, Emerson
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Educação Física
• País: BR
• Palavras-chave em Português: Corredores (Esportes); Corridas; Aptidão física; Atrofia muscular; Antioxidantes; Stress oxidativo
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA
• Link de acesso: https://ri.ufs.br/handle/riufs/4930

During the last two decades it has been substantially growing interest in anaerobic performance of children and adolescents. However, few studies address the effects of high intensity and short duration exercise, such as RAST TEST, on oxidative stress and indirect markers of muscle injuries in adolescents, which can promote the proper management of training sessions and, consequently, Athletic performance. In this sense, the objective of this study was to investigate the acute effect of a maximum anaerobic testing - RAST TEST - on oxidative stress and muscle damage in adolescents corridors. To do this voluntarily participated in the study nine runners adolescents of both sex and age between 15 and 18 years (59.25 ± 11.45 kg; 1.70 ± 0.06 m; 19.57 ± 2.52 kg. m2, 0.84 ± 12:08 and 12.56 ± 4.01% G). All volunteers performed a week of familiarization, being respected a minimum of 72 hours to carry out the RAST TEST itself. Blood collection to perform biochemical analyzes were performed before and after the test. Data were expressed as mean ± SD and analyzed using the Student t test for paired data and applied Pearson correlation between all variables, adopting a 5% significance level. Serum lactate dehydrogenase (LDH) (before: 326.0 ± 72,65U / L and powders: 758.72 ± 135,09U / L) and creatine kinase (CK) (before: 278.1 ± 78 64U / L and post: 983.62 ± 339.49 U / L) increased by 132.72% and 253.69% after the test RAST (p ≤ 0.05). Regarding the antioxidant enzyme, the protocol also increased significantly (p ≤ 0.05) glutathione activity after the test, and demonstrated increased oxidative stress assessed by TBARS in the post-test (p ≤ 0.05). According to the presented results we conclude that the RAST TEST addition to properly assess the anaerobic fitness of adolescent athletes, promoting a significant increase in muscle damage markers and increased concentrations of TBARS and GPx activity. However, the sharp increase in GPx activity suggests an efficient mechanism for the prevention of cell damage and thus preventing oxidative stress in adolescents athletes the RAST test. Key words: high-intensity exercise, anaerobic test, athletic performance, antioxidant enzymes, muscle damage markers.