Extração e microencapsulação de compostos bioativos presentes nas diferentes variedades das própolis brasileiras

• Ano de defesa: 2017
• Autor(a) principal: Andrade, Julianna Karla Santana
• Orientador(a): Narain, Narendra
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Programa de Pós-Graduação: Pós-Graduação em Ciência e Tecnologia de Alimentos
• Palavras-chave em Português: Própole; Compostos bioativos; Extração de própolis; Antioxidantes; Própolis; LC-MS/MS; Microencapsulação
• Palavras-chave em Inglês: Bioactive compounds; Microencapsulation
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
• Link de acesso: http://ri.ufs.br/jspui/handle/riufs/16106

Propolis has attracted interest in recent decades due to the various functional properties attributed to the presence of bioactive substances, which serves for improving health and preventing diseases. The aim of the present study was to determine the content of bioactive compounds present in different Brazilian propolis varieties of the state of Alagoas and Sergipe: brown (Hyptis divaricata), green (Baccharis dracunculifolia) and red (Dalbergia ecastophyllum), through different extraction methods and to obtain dried powders from the extracts of the propolis by means of the spray-drying atomization process. The content of phenolic compounds, flavonoids and antioxidant activity (DPPH, ABTS+, FRAP, ORAC) were determined in extracts and microparticles of propolis. The chromatographic profile of extracts and microparticles was obtained from the Ultra Fast Liquid Chromatography system. Using the LC-MS/MS system, in the MRM mode, the identification and quantification of the bioactive compounds present in the samples of the extracts and microparticles of the propolis were carried out. In addition, microparticles were evaluated for their morphology (SEM, particle size, XRD, FTIR), physico-chemical characterization (moisture, water activity, solubility and hygroscopicity), encapsulation efficiency and retention of bioactive compounds. The results showed that the extracts and the microparticles of propolis presented a high content of total phenolics, flavonoids and antioxidant activity. Maltodextrin and gum arabic proved to be efficient drying aid, imparting good encapsulation efficiency (70-79%). The microparticles presented spherical forms with extremely smooth surfaces, with amorphous characteristics, low water activity, humidity and hygroscopicity, high solubility and good retention of the bioactive compounds. As for the identification and quantification of the phenolic compounds present in the samples, the richness of the chemical composition is verified, which brings prespective for the use of extracts and microparticles in pharmaceutical formulations and in the elaboration of new functional foods.