Integrative biology for prospecting markers of Gall Wasp resistance in Eucalyptus

• Ano de defesa: 2023
• Autor(a) principal: Calazans, Crislaine Costa
• Orientador(a): Silva-Mann, Renata
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Programa de Pós-Graduação: Pós-Graduação em Agricultura e Biodiversidade
• Palavras-chave em Português: Eucalipto; Vespas; Marcadores genéticos; Terpenos; Bioinformática; In silico; ISSR
• Palavras-chave em Inglês: Leptocybe invasa; Transposons; Bioinformatics; Terpenes; transposable; Elements
• Área do conhecimento CNPq: CIENCIAS AGRARIAS
• Link de acesso: https://ri.ufs.br/jspui/handle/riufs/18674

In Brazil, the gall wasp, Leptocybe invasa, causes damage to Eucalyptus production. The objectives of this thesis were to study L. invasa in Eucalyptus genotypes and identify genes and metabolic pathways related to plant-insect interactions. In addition, in an effort to contribute to the control of this pest, genotypes were characterized in terms of resistance to the gall wasp, using transposable elements and ISSR markers. The study was divided into three stages: i) meta-analysis of gene associations to combat L. invasa; ii) in silico analysis; and iii) phenotypic and genotypic analysis of Eucalyptus genotypes. For the meta-analysis, the search terms "Leptocybe invasa" OR "gall wasp Eucalyptus" were used in articles from the scientific databases Scopus and Web of Science, using the R Bibliometrix package. Annotation information from E. grandis in Phytozome v7.0 was validated. Regarding metabolic pathways, the Plant Reactome Gramene Pathways database was employed and technological innovations in the form of patents were searched using the Orbit Intelligence® software. Conserved sequences of transposable elements of the Eucalyptus spp. genome were identified by in silico analyses. Resistant and susceptible genotypes were differentiated according to the gall wasp damage level, by means of image analysis with Groundeye® equipment. The genotype response to L. invasa was discriminated based on transposable element sequences and ISSR markers. The meta-analysis included 234 documents; 116 from the Web of Science and 118 from Scopus. Metabolic pathway analysis detected a pathway associated with the Eucgr.B02310 gene, related to the presence of terpenes. Most of the surveyed patents deal with pesticide control of L. invasa. In silico analysis identified conserved regions in the Eucalyptus spp. genome, associated with the production of secondary metabolites such as terpenes. The phenotypic analysis effectively distinguished gall-wasp susceptible from -resistant genotypes. Transposable elements and ISSR markers generated sufficient fragments to differentiate resistant from susceptible genotypes. These results contribute to the management of L. invasa in Eucalyptus and highlight the importance of combining biological, chemical and genetic approaches for this purpose.