Análise da associação entre polimorfismos genéticos e a sintomatologia característica de exposição a agrotóxicos em trabalhadores rurais

• Ano de defesa: 2017
• Autor(a) principal: Telles, Alysson Fellipe Costa
• Orientador(a): Kaiser, Claudia Cristina
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Programa de Pós-Graduação: Pós-Graduação em Ciências Aplicadas à Saúde
• Palavras-chave em Português: Saúde e trabalho; Camponeses; Colinesterases; Organofosforado; Saúde Ocupacional; Polimorfismos Genéticos; Butirilcolonesterase; Paraoxonase
• Palavras-chave em Inglês: Organophosphates; Occupational Health; Genetic Polymorphisms; Butyrylcholinesterase; Paraoxonase
• Link de acesso: http://ri.ufs.br/jspui/handle/riufs/7179

Introduction: Cholinesterases and Paraoxonase 1 are mediators of poisoning process by organophosphate (OP). Monitor the activities of these enzymes and know the genetic variability of the agricultural population is of great importance in the evaluation of possible risk groups for poisoning OP. Objective: This case-control study aimed to investigate the association of genetic markers (tag SNPs) in BchE and PON1 genes with characteristic symptoms of exposure to OP pesticides in rural workers.Methodology: 427 patients, both sexes, mean age 40.96 years old, divided into 226 with and 201 without characteristic symptomatology of exposure to pesticides, were genotyped for three tag SNPs, rs1803274 (BChE gene), rs662 e rs854560 (PON1 gene). Also, socio-demographic and economic parameters were analyzed. BChE activity was demonstrated, as well as the profile of workers' health was evaluated. For the test of association of the categorical variables, the Chi-square test and Fisher's exact test. For the genetic models tests the Binary Logistic Regression was used in the Additive Model and Fisher's Exact Test and Chi-SquareTest in the Dominant and Recessive Models (p < .05). Results: Associationstatistically significant for place of residence with symptoms presentation; between the rs1803274 and symptoms, in the Recessive model. Muscular weakness was the most representative symptom, presenting a statistical association with rs1803274, in the Additive and Dominant model. BChE activity was associated with rs662 in theRecessive model, demonstrating that individuals with this SNP have a higher chance of presenting reduced activity for this enzyme. Conclusion: The results demonstrate that the intrinsic factors (SNPs) and extrinsic (residence) studied can modulate theprocess of intoxication by OR, increasing or reducing the susceptibility of the individuals involved.