Diversidade e estrutura genética de populações naturais de Erythrina velutina Willd

• Ano de defesa: 2010
• Autor(a) principal: Melo, Marília Freitas de Vasconcelos
• Orientador(a): Silva-Mann, Renata
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Biotecnologia de Recursos Naturais
• País: BR
• Palavras-chave em Português: Parâmetros genéticos; Heterozigosidade; Diversidade
• Palavras-chave em Inglês: Genetic parameters; Heterozygosity; Diversity
• Área do conhecimento CNPq: CNPQ::OUTROS
• Link de acesso: https://ri.ufs.br/handle/riufs/3300

A study based in DNA and isozyme markers was carried out to evaluate the diversity and genetic structures of natural populations of Erythrina velutina Willd., aiming monitored prospection of the genetic variability for two populations from the Atlantic Forest (Santana do São Francisco-SE) and Caatinga (Pinhão-SE) Biomes. Young leaves of twenty individuals per population were sampled from each population. Primers of ten arbitrary bases sequence and fifteen enzymatic systems were used. In RAPD analysis, the population from Atlantic Forest Biome originated 100 polymorphic loci and population from Caatinga Biome, 112 loci. It was observed that the genetic structure for population from Caatinga present higher number of observed and effectives alleles, which implicate in a higher heterozigozity. The observed average heterozygozity was higher than the expected heterozigozity by Hardy- Weinberg, which indicate a excess of heterozygotes, and this value might be confirmed by negative value of the Wright´s fixation index (-0.5098). In relation to genetic diversity between populations (Fst and Gst) the values were similar for both markers. The results suggest the use of M1, M7, M11 and M14 individuals from Atlantic Forest Biome; and the M5, M6, M18 and M19 individuals from Caatinga Biome as most divergent for future chemical, biochemical and pharmacological studies.