Detalhes bibliográficos
| Ano de defesa: |
2016 |
| Autor(a) principal: |
Ávila, Dayara Virgínia Lino
 |
| Orientador(a): |
Passos, Elisangela de Andrade |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Sergipe
|
| Programa de Pós-Graduação: |
Pós-Graduação em Química
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
https://ri.ufs.br/handle/riufs/6051
|
Resumo: |
In this work, four methods were developed for analysis of feed for dogs and cats using atomic spectrometry techniques. Initially, the multivariate optimization of sample digestion procedure was employed in wet feed for dogs and cats and then the simultaneous determination of macroelements (Ca, K, Mg and P), microelements (Cu, Fe, Mn, and Zn) and trace elements (Al, B, Ba and Sr) was carried out by optical emission spectrometry with inductively coupled plasma (ICP OES). The results of the elements concentrations in the samples were in agreement with the values provided and regulated by Ministry of Agriculture, Livestock and Supply (Ministério da Agricultura, Pecuária e Abastecimento - MAPA) and American Association of Official Food Control Officials (AAFCO). Limits of detections (LoD) ranged between 0.1 μg g-1 (Ba, Mg and Sr) and 15.0 μg g-1 (P) and limits of quantification (LoQ) between 0.3 μg g-1 (Ba, Mg and Sr) and 49.9 μg g-1 (P). In the second part of the work two analytical methods were optimized for Co and Cr determination in wet food (sachets and cans) for dogs and cats using direct sample analysis (SS) and detection by high resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GF AAS). Pyrolysis temperatures were 1500 ° C and 1400 ° C for Cr and Co determination, respectively. Atomization temperatures of 2500 ° C for Cr, and 2400 ° C for Co with chemical modifiers were adopted. The optimized method for Co and Cr determination in wet feed was fast, precise and accurate and contributes to the concept of “Green Chemistry”. The results obtained for concentrations of Cr and Co were not compared to any official rule, since there is no regulation for these elements. The LoD's obtained for Cr and Co were 0.01 and 0.05 ug g-1, respectively, and LoQ's obtained were 0.03 to 0.17 ug g-1 for Cr and Co, respectively. Finally, in the third part of the work, an analytical method for determination of Zn in dry feed for dogs and cats using energy dispersive Xray fluorescence spectrometry (EDXRF) was developed. Sample were prepared as pressed pellets. The concentration of Zn in seven dry feed samples for dogs and cats were determinate. The results were statistically compared to the values found by the analytical method by ICP OES. Additionally, paired t-test between concentrations of Zn by EDXRF and ICP OES was applied. The LoD e LoQ obtained for Zn determination were 0.1 e 0.4 mg kg-1, respectively. The independent methods were not significantly different at a 95% confidence level. In our work, the analytical methods proposed were efficient, fast and reliable for the determination of macroelements (Ca, Mg, K and P), microelements (Cu, Fe, Mn and Zn) and trace elements (Al, B, Ba, Co, Cr and Sr) in feeds for dogs and cats using atomic spectrometry techniques. This work was financially supported through the project Universal notice nº 014/2013 approved by CNPq, registered process nº 482416 / 2013-0. |
