Desenvolvimento de metodologia para avaliação de resíduos de agrotóxicos em café torrado

• Ano de defesa: 2015
• Autor(a) principal: Souza, Nicaellen Roberta da Silva
• Orientador(a): Navickiene, Sandro
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Química
• País: BR
• Palavras-chave em Português: Química analítica; Café > Cultivo; Produtos químicos agrícolas; Pesticidas; Café torrado; Coffea arabica; Agrotóxicos; Sonicação; Ultrassom; LLE; HPLC/UV-DAD; UPLC/UV-DAD; LC-MS/MS
• Palavras-chave em Inglês: Sonication; Pesticides; Roasted coffee; Coffea arabica; LLE; HPLC/UV-DAD; UPLC/UV-DAD; LC-MS/MS
• Área do conhecimento CNPq: CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: https://ri.ufs.br/handle/riufs/6124

Agriculture is one of the main economic activities of the Brazil, possessing the culture of coffee as their main primary commodity. In order to achieve high levels of production and prevent the loss of crops, farm inputs such as pesticides are used in large quantities. This type of practice and the increased public concern consumer are motivating scientific research about these topics, with the aim of ensuring food safety of products supplied for consumption. However, the literature does not report methodologies for the determination of pesticide residues in roasted coffee, with the methods of extraction and analysis proposed, which according to bibliographic data present residues of these substances even after roasting. In this context, the present study sought to develop an analytical methodology to determine carbofuran, cypermethrin, chlorpyrifos, clothianidin, disulfoton, endosulfan, spirodiclofen, haloxyfop, imidacloprid, tebuconazole, triadimenol and triadimefom in roasted coffee using solid liquid extraction method by sonication with clean-up step through liquid liquid extraction (LLE) and instrumental analysis for ultra performance liquid chromatography tandem mass spectrometry detector (LC-MS/MS). For both, the optimization of chromatographic conditions and assessing the best method of clean-up was carried out. With that, the conditions for better quantitative response was obtained with 0.5 g of roasted coffee, 5 mL extraction solvent by sonication, 1 mL of water and 1 mL of dichloromethane in the clean-up by LLE. During the validation procedure in LC-MS/MS positive matrix effect was observed for all analytes except of cypermethrin in which there was suppression of the signal by array, causing, prepared in the curve-based extract, recoveries between 74.2%-102.4% standard relative deviation (SRD) between 0.6% and 10.2%, in levels concentration assessed in the process, Furthermore, the linearity obtained was in the range of 0.9979 to 0.9998 for pesticides , carbofuran, disulfoton, imidacloprid, clothianidin tebuconazole, triadimenol, triadimefom and ensuring the efficiency of the methodology.