Toxicidade aguda e atividade antinociceptiva do extrato aquoso e óleo essencial da Hyptis pectinata (L.) Poit (sambacaita), em modelo murino de artrite induzida por cristais de urato de sódio

• Ano de defesa: 2006
• Autor(a) principal: Lira, Amintas Figueirêdo
• Orientador(a): Antoniolli, Ângelo Roberto
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Ciências da Saúde
• País: BR
• Palavras-chave em Português: Hyptis pectinata L. Poit.; Toxicidade aguda; Antinocicepção; Artrite gotosa
• Palavras-chave em Inglês: Hyptis pectinata L. Poit; Acute toxicity; Antinociception; Gout arthritis
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA
• Link de acesso: https://ri.ufs.br/handle/riufs/3709

Hyptis pectinata L. Poit. (Lamiaceae), widely known as Sambacaitá, is an example of a plant commonly used in folk Medicine; various biological effects are associated with its extracts, such as: analgesic, anti-inflammatory, and anti-microbial. Many studies in vivo and in vitro has assessed the effects promoted by the aqueous extract and essential oil of the leaves of the plant, whose chemical composition constitute their water soluble compounds and their volatile compounds, respectively. This study aimed at keeping on these studies, determining the chemical constituents and the acute toxicity of the sample of the aqueous extract and essential oil used to study the analgesic effect of these extracts in an acute articular inflammatory condition, similar to an episode of acute arthritis as in Gout. The phyto-chemical studies of aqueous extract identified the presence of alkaloid, tannin and flavonoid. The analysis of the constituents of the essential oil was made by gas chromatography and mass spectrometer, showing as main constituents: β- cariofilene and Germacrene-D. The evaluation of acute toxicity was represented by the establishment of DL50 in Swiss mice, obtained by the probits method, it was not possible to measure the DL50 of the aqueous extract due to its low toxicity, as for the essential oil, it was obtained a 1.1 g/kg DL50. The analgesic effect was studied using the sodium urate crystals induced arthritis model, in Wistar mice, as proposed by Coderre and Wall; the aqueous extract presented expressive analgesic effect with the 50 mg/kg doses, which was reverted by the opioid antagonist, naloxone (5 mg/kg). The essential oil did not present analgesic effect with the tested doses. The results showed that the aqueous extract has low toxicity and it presents analgesic effect in a rheumatic condition, similar to Gout arthritis, while the essential oil presents medium toxicity and it does not show analgesic effect in the proposed rheumatic condition.