Nanopartículas lipídicas contendo tacrolimo para uso tópico : desenvolvimento e caracterização

• Ano de defesa: 2015
• Autor(a) principal: Dantas, Isabella Lima
• Orientador(a): Lira, Ana Amélia Moreira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Ciências Farmacêuticas
• País: Brasil
• Palavras-chave em Português: Dermatite atópica - tratamento; Nanopartículas lipídicas; Tacrolimo
• Palavras-chave em Inglês: Lipid nanoparticles; Tacrolimus; Atopic dermatitis
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: https://ri.ufs.br/handle/riufs/3948

The Atopic Dermatitis (AD) is a chronic inflammatory skin disease that affects patients with disabilities in the skin barrier and low immune response. Tacrolimus ointment for topical use is an immunomodulator that has shown to be a good alternative for the treatment of AD. However, it also has adverse effects such as low and variable bioavailability, burning sensation and itching at the application site. Thus, the development of new dosage forms that overcome these drawbacks is crucial to the success of the therapy. The objective of this study was to obtain lipid nanoparticles (LN) of tacrolimus for future application in AD therapy. The LN were obtained by solvent diffusion method associated with ultrasonication using stearic acid (SA) and beeswax as solid lipid in the solid lipid nanoparticles (SLN) and oleic acid (OA) incorporated into the solid lipid matrix in the carriers lipid nanostructured (NLC). Lipid dispersions were characterized by determining the particle size, Polydispersity Index (PDI), Zeta Potential (ZP) and and drug content in the NL. Evaluation by Transmission Electron Microscopy (TEM), analysis by Differential Scanning Calorimetry (DSC), X-ray diffraction (XRD) and Infrared Spectroscopy in the region Fourier Transform (FT-IR). As results, LN presented nano-sized spherical shape with an average diameter ranging from 139 – 274, 9 nm and IPD from 0.3 - 0.5. PZ of LN was higher than -|25 mV|, which ensures the stability of the dispersion. In the results of DSC, it was observed that the endothermic event in the NLC was shifted to lower temperatures. Drug loading of 2,3 a 3,2%. The results of XRD also showed low crystallinity when NLC was compared with SLN. The results of FTIR was not observed interaction between the drug and the lipid components of the matrix. Then, it can be concluded that NL develop were successfully obtained and may represent promising system for the placement of tacrolimus in topical formulations.