Desenvolvimento de um sistema de medição em tempo real, por espectroscopia de bioimpedância, capaz de determinar a concentração celular

• Ano de defesa: 2020
• Autor(a) principal: Silva, Marcos Antonio Salvino da
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Rio de Janeiro; Brasil; Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa de Engenharia; Programa de Pós-Graduação em Engenharia Biomédica; UFRJ
• Palavras-chave em Português: Bioimpedância; Bacillus thuringiensis; Saccharomyces cerevisiae; Bioprocesso; Engenharia Biomédica
• Link de acesso: http://hdl.handle.net/11422/21499

This work developed an alternative biomass measurement system applied for real-time monitoring (online) of bioprocesses, through bioimpedance spectroscopy, using the BIS-STEP technique. The construction of this work was carried out in two stages: the first, to test the feasibility of applying this bioprocess monitoring technique, the experiments were carried out with the commercial yeast Saccharomyces cerevisiae. In this step, use a linear regression multivariate (LRM) to estimate the total number of total and viable cells, using bioimpedance parameters. The second step, in the interest of Farmanguinhos/Fiocruz, to extrapolate this technique to bacteria Bacillus thuringiensis var. israelensis (Bti). The latter (Bti) presented greater difficulty in the direct application of LRM commonly used in dielectric spectroscopy or bioimpedance techniques. A solution found, using respiratory parameters - oxygen uptake rate (OUR), shows the most comprehensive ones and can also be applied to the data obtained from the first experiments. The statiscal results were satisfactory for both microorganisms with an average Pearson correlation coefficient (r) above 0.9 and the Bland-Altman model evaluated the agreement between analytical and bioimpedance methods.