Detalhes bibliográficos
| Ano de defesa: |
2012 |
| Autor(a) principal: |
Jacometo, Carolina Bespalhok |
| Orientador(a): |
Dionello, Nelson José Laurino |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Pelotas
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Zootecnia
|
| Departamento: |
Faculdade de Agronomia Eliseu Maciel
|
| País: |
BR
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
https://guaiaca.ufpel.edu.br/handle/123456789/2618
|
Resumo: |
Dietary polyunsaturated fatty acids (PUFAs) are negative regulators of hepatic lipogenesis and their effects are at transcriptional levels. The essential fatty acids omega-3 and omega-6, consumed during pregnancy can benefit maternal and offspring health. The nuclear receptor peroxisome proliferator-activated receptor α (PPARα), when activated by PUFAs enhances lipolytic genes. Liver X receptor α (LXRα) and sterol regulatory element binding protein 1c (SREBP-1c) are nuclear receptors that enhance lipogenic genes, and are repressed by PUFAs. The present study aimed to investigate the effects of diets, rich in omega-3 or omega-6, consumed throughout three generations, on biochemical parameters and the expression level of some genes related to lipid metabolism. For this study we used adult Wistar/UFPel rats, composing the founding generation, females that received diet with flaxseed oil (OM group, n=18) or soybean oil (CTL group, n=18) during pregnancy. At F1 weaning, 48 females offspring were selected and allocated in three groups, females from OM group, that continued receiving diet with flaxseed oil (OM/OM group, n=16), females from OM group, that started to receive diet with soybean oil (OM/CTL group, n=16) and females from CTL group, that continued receiving diet with soybean oil (CTL/CTL group, n=16). At F2 weaning, 16 females offspring of each group were selected and continued receiving the same diets (OM/OM/OM group, OM/CTL/CTL group and CTL/CTL/CTL group). The females were evaluated in the pre-partum (19±1 days of pregnancy) and at postpartum period (21 days after parturition). The dietary fatty acid profile did not affect the diet daily intake and the pregnancy rate (P>0.05), however, dams that received the diet rich in omega-3 had heaviest offspring (P=0.01). Diets with higher omega-3 level decreased triglycerides serum levels, and maintained constant the non-esterified fatty acids (NEFA) levels (P=0.04), while the animals fed with high proportion of omega-6 had fluctuant NEFA levels, and an outstanding increase in the postpartum period of F2 generation (P=0.02). PPARα, RXRα, LXRα and SREBP-1c had been regulated by the diets throughout generations. In the pre-partum moment of F2 generation, the animals fed with high omega-3 had a lower expression of LXRα (P=0.01) indicating its effectiveness on inhibiting lipogenic action. Our results indicate that the PUFAs effect on the control of lipolysis and lipogenesis is cumulative throughout generations and the omega-3 exerts a better control. |
