Detalhes bibliográficos
| Ano de defesa: |
2011 |
| Autor(a) principal: |
Prestes, Luciana de Souza |
| Orientador(a): |
Meireles, Mario Carlos Araújo |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Tese
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal de Pelotas
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Veterinária
|
| Departamento: |
Veterinária
|
| País: |
BR
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
|
| Área do conhecimento CNPq: |
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| Link de acesso: |
https://guaiaca.ufpel.edu.br/handle/123456789/2575
|
Resumo: |
The use of medicinal plants in treatment of disease follows the history of mankind. In third world countries, including Brazil, where 70-80% of the population lacks health pharmaceutical, medicinal plants are used as alternative therapy. To evaluate the antimicrobial activity in vitro and in vivo, extracts of plants of the family Myrtaceae against microorganisms that cause diarrhea and its effect on intestinal motility through biological model in rats. We selected three plant family Myrtaceae popularly used in the treatment of diarrhea: guava (Psidium guajava L.), surinam cherry (Eugenia uniflora L.) and strawberry guava (Psidium cattleyanum Sabine) belonging to the COOPAVA (Cooperative Agricultural Production Vista Alegre). Hydroalcoholic extracts were prepared with essential oils and leaves of plants harvested at three phenological stages: fruit set, flowering, and rest. The extracts were tested for their antimicrobial activity against bacteria (Escherichia coli, Salmonella typhimurium and Staphylococcus aureus). Besides the hydroalcoholic extracts prepared essential oils from three plants and tested against the same microorganisms and some of its constituent were identified by gas chromatography coupled to the spectrometer massas-GC/MS. The hydroalcoholic extract of guava and infused with a better outcome in vitro test was chosen for the evaluation of antidiarrhoeal activity of the plant in vivo. In vitro tests were performed by broth microdilution technique for assessment of minimal bactericidal (MBC). The in vivo tests were performed by testing for induction of diarrhea with castor oil in rats. The constituents found in all plants by chromatographic analysis were α-and α-humulene copaene. The best result of the hydroalcoholic extract against S. aureus was prepared with fresh guava leaves harvested during the flowering season with geometric mean of quadruplicates of 3.25%, against S. typhimurium the three plants showed antibacterial activity at concentration of 25% with extracts harvested at the time of fruiting and leaf dry weight (strawberry guava and surinam cherry) and at home with fresh and dry leaf of guava.E. Front coli, the minimum bactericidal concentration was 25% with the leaf extract surinam cherry harvested in dry home, dry leaves harvested at the time of strawberry guava fruit and dried leaves of guava harvested in the home. The oil of guava showed bactericidal activity against E. coli at a concentration of 2%, compared to S. aureus and S. typhimurium at a concentration of 8%. The oil of guava showed no bactericidal activity against microorganisms tested and the oil surinam cherry showed bactericidal activity at a concentration of 8% compared to S. aureus and S. typhimurium. With our method, the hydroalcoholic extract and the infusion of the dried leaves harvested at the fruiting of guava, showed no activity antidiarrhoeal. |
