Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5

• Ano de defesa: 2014
• Autor(a) principal: Araujo, Itauá Leston
• Orientador(a): Leite, Fabio Pereira Leivas
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Pelotas
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biotecnologia
• Departamento: Biotecnologia
• País: BR
• Palavras-chave em Português: Adjuvante oleoso; Pichia pastoris; ELISA; Alfaherpesvírus; Vacina de subunidade
• Palavras-chave em Inglês: Oil adjuvant; Pichia pastoris; ELISA; Alphaherpesviruses; Subunit vaccine
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA
• Link de acesso: http://guaiaca.ufpel.edu.br/handle/123456789/1235

Glycoprotein D (gD) is essential for attachment and penetration of bovine herpesvirus type 5 (BoHV-5) into susceptible cells, and is a major target of host immune systems, inducing strong humoral and cellular immune responses. The immunogenicity of recombinant BoHV-5 (rgD5) expressed in Pichia pastoris was evaluated in bovines. Vaccines formulated with 100 μg rgD5 dose and adjuvants (Montanide ISA50V2 or Aluminum Hydroxide) with or without inactivated BoHV-5 or rgD5 without adjuvants were administered intramuscularly. A commercial vaccine was also used as control. The vaccine formulation rgD5 + ISA50V2 stimulated humoral immune responses after two doses, and higher titer of neutralizing antibodies were obtained in all three oil-based adjuvants formulations when compared to Hydroxide Aluminium adjuvant or the commercial vaccine. The vaccine BoHV-5 + rgD5 + ISA50V2 stimulated titers of neutralizing antibodies approximately 8 log2, which demonstrated higher correlations on the Indirect ELISA. Together, the results suggest that the recombinant gD5 conserved neutralizing epitopes and was able to stimulate a efficient humoral immune response in bovines.