Caracterização morfológica e molecular para auxílio no melhoramento genético em gérbera

• Ano de defesa: 2012
• Autor(a) principal: Benemann, Daiane de Pinho
• Orientador(a): Peters, José Antônio
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Pelotas
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biotecnologia
• Departamento: Biotecnologia
• País: BR
• Palavras-chave em Português: EST-SSR; Caracteres morfológicos; Correlação; Gerbera spp.
• Palavras-chave em Inglês: EST-SSR; Morphological characters; Correlation
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL
• Link de acesso: http://guaiaca.ufpel.edu.br/handle/123456789/1205

Gerbera is one of the most important plants in the global ornamental market. Due to fierce competition in this market, obtaining different genotypes is necessary. Therefore, information on the genetic variability of this species may help breeding programs in order to select new promising genotypes. This study aimed to develop EST-SSR markers for gerbera, and to check the genetic variability among accessions by multivariate analysis using morphological (qualitative and quantitative), and molecular markers. Also to analyze the correlation between morphological and molecular markers to elucidate the genetic variability of Gerbera spp.. As a result, 17 EST-SSR markers were developed for use in studies of molecular characterization. These were highly polymorphic and allowed the differentiate of all gerbera accessions. The analysis of morphological (quantitative and qualitative) characters, by means of multivariate analysis and principal components enabled the delection of a wide genetic variability among accessions of gerbera. The correlation analysis between morphological characters show that there is some influence on other characters. The analysis of molecular and morphological data, using the sum of the dissimilarity matrices, it was possible to obtain a better grouping of the accessions studied. The results of this study may assist gerbera breeding programs in the selection of parental genotypes for controlled crossings.