Transposons e retrotransposons como marcadores moleculares em plantas

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Santos, Gabriela Guerra dos
Orientador(a): Oliveira, Antônio Costa de
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Pelotas
Programa de Pós-Graduação: Programa de Pós-Graduação em Biotecnologia
Departamento: Biotecnologia
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://guaiaca.ufpel.edu.br/handle/123456789/1208
Resumo: The breeding programs have made significant contributions to obtain more productive genotypes. Studies in Plant biotechnology has been one of the tools to aid plant breeding, as well as the use of molecular markers. Transposons and Retrotransposons are ubiquitous in plant genomes and are increasingly studied. This study aimed to evaluate the genetic similarity among different rice cultivars using the techniques of IRAP and REMAP using the presence of retrotransposons. To verify this similarity was performed in 20 rice genotypes (Brazilian, Japanese and Filipinos) a DNA extraction for subsequent PCR amplification using the techniques IRAP and REMAP. Analysis of amplification products was made by classifying the fragments as independently presence and absence of amplification at different heights polyacrylamide gel for data were used to construct a binary matrix. The data generated were used for the calculation of genetic similarity between all pairs of individuals with the aid of the computer program NTSYS pc 2.1. To describe the patterns of similarity was adopted Dice coefficient and a dendrogram was constructed by the method of unweighted UPGMA grouping in pairs. Furthermore, was estimated cophenetic correlation coefficient (r), according to the Mantel test and statistical stability of the grouping was estimated by analysis of bootstrapping with 1000 replicates through the computer program WinBoot 1.0. The results of this work show that it is possible to assess the genetic variability in rice genotypes using IRAP and REMAP techniques through the use of retrotransposons as molecular markers.