O padrão glicêmico sanguíneo interfere no metabolismo de biofilmes de candida sobre superfícies abióticas?

• Ano de defesa: 2019
• Autor(a) principal: Muniz, Isis Morais Bezerra
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso embargado
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Odontologia; Programa de Pós-Graduação em Odontologia; UFPB
• Palavras-chave em Português: Candida; Biofilmes; Saliva; Diabetes Mellitus; Biofilms; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/20316

Candida is the main fungal pathogen in humans, and the species albicans is the most prevalent in oral infections, such as oral candidiasis. In immunocompromised individuals, those with Diabetes Mellitus, C. albicans can proliferate on mucous and abiotic surfaces, promoting bigger susceptibility to other local and/or systemic infections. The deficit of glycemic control in these patients can promote alteration of the biofilm pathogenicity, making candidiasis more prevalent, predisposing these individuals to several alterations in the oral cavity. Thus, the influence of blood glycemic patterns on the development of Candida albicans biofilm (ATCC 90028) on abiotic surfaces of titanium and acrylic resin was evaluated. Stimulated salivary collection was performed from individuals with pre-established bloody glycemic conditions and concentrations, considering normoglycemic individuals (≤120 mg / dL) - NG and hyperglycemic individuals (> 120 mg / dL) - HG. Subsequently, the saliva was filtered and used for the cultivation of C. albicans biofilms (n = 12 / group) on these surfaces, which were analyzed at 24 and 72 hours of cultivation. Of the 95 salivary samples collected, 49 were collected from hyperglycemic patients and 46 normoglycemic patients. Saliva donors were characterized and biofilm analyzed for cell viability (CFU/mL), Total Protein dosage (PT), phospholipase activity and biofilm morphology by fluorescence optical microscopy with Calcofluor White ( CFW). Statistical analysis of the numerical variables was performed using the Mann-Whitney test (-5%) and the biofilm morphology determined qualitatively. The sample of saliva donors (n = 95) was composed of 40 males and 55 females, with a mean age of 51.3 (±11,2) years. When compared between groups (NG x HG) and between times (24 x 72h) there was no statistically significant difference in relation to any of the evaluated parameters. For the results on acrylic resin surfaces the CFU/mL count determined medians of 6.4 and 6.5 for 24 and 72 h analysis respectively, with no difference (p>0,05) between groups (NG x HG). The PT production determined from medians 0.3 g/dL and 2.1 g/dL for 24 and 72 h analysis, respectively, with no difference between groups (NG x HG). Phospholipase activity determined medians of 0.6 mm for both 24 and 72 h analyzes, with no difference between groups (NG x HG). For the results on titanium surfaces the CFU/mL count determined medians of 6.5 for the 24 and 72 h analyzes. The PT production determined from medians 0.7 g/dL and 0.2 g/dL for 24 and 72 h analysis respectively. Phospholipase activity determined medians of 0.6 mm for both 24 and 72 h analyzes, with no difference between groups (NG x HG) for all analyzes cited. Blood glycemic concentrations did not influence C. albicans biolfilm metabolism on abiotic surfaces. The results of the present study may be relevant for the development of new therapies aimed at controlling C. albicans infections and their diseases.