Avaliação de fontes de metionina e dietas com perfil de aminoácido total e digestível para codornas japonesas.

• Ano de defesa: 2017
• Autor(a) principal: Lucena, Patrícia Barbosa de Lacerda
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Zootecnia; Programa de Pós-Graduação em Zootecnia; UFPB
• Palavras-chave em Português: Zootecnia.; Biodisponibilidade.; Crescimento.; Desempenho.; Digestibilidade.; Ovos.; Postura.; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/18977

The emergence of synthetic amino acid production has brought several advantages to poultry production systems, from performance till production costs. This has attracted the attention of researchers for the application of new methodologies for formulating feeds based on the specific needs of essential amino acids (EAA), among them the formulation of diets based on digestibility or in the future in the bioavailability of EAA. Thus, the objectives of this work were to evaluate the relative bioavailability (RB) of Methionine Hydroxy-Analog (DL-MHA) compared to DL-Methionine (DL-Met), and to compare diets formulating based on total versus digestible amino acids in the japanese quail (Coturnix coturnix japonica) feeding. This thesis was divided into two Chapters. In Chapter I, 550 female of japanese quails, distributed in a completely randomized experimental design, were used in a 2x5 + 1 factorial scheme, two methionine sources (DL-Met and DL-MHA) supplied in 5 levels (0, 29, 0.35, 0.41, 0.47 and 0.53%) plus a control diet, without inclusion of synthetic methionine sources, totaling 11 treatments, with five replicates of 10 birds per experimental unit. The control treatment consisted of an iso-energetic and isoprotein diet based on corn and soybean meal, without supplementation of methionine recommended for Japanese quails from 1 to 14 days of age and supplemented with DL-Met (99%) or DL-MHA (88%) in expense of glutamic acid. Weight gain (kg) and feed conversion (kg/kg) were assessed at 7 and 14 days of age. Analysis of variance was performed (P<0.05) and BR was calculated by slope ratio technique by the relationship between the multiple regression coefficients for MHA-FA by the regression coefficient for DLMet. For weight gain, feed conversion and feed efficiency from 1 to 7 day, BR of MHA-FA was 90.61, 75.00% and 70.19% (R² = 0.98, 0.85 and 0.96). For weight gain, feed conversion and feed efficiency from 1 to 14 day, BR of MHA-FA was 91.44, 95.04 and 87.46% (R² = 0.98 e 0.85). From 1 to 14 d, estimates were de 91.44 e 95.04% (R² = 0,95 e 0,92), respectively. The average BR of MHA-FA for Japanese Quails was 78.6 % from 1 to 7 d and 85.55 % from 1 to 14 d. Quails fed basal or MHA-supplemented diets showed lower villus height and considerable increase in the number of mitoses in intestinal crypts when compared to those receiving DL-Met. The increase in methionine levels above 0.43% favored a reduction in the rate of mitosis in crypt cells. The evaluation of the estimated methionine level for the best performance of Japanese quail from 1 to 14 days of age is 0.47%. The best performance of japanese quails from 1 to 14 days of age was with the level of 0.47% of total Met or 0.43% of digestible Met. In Chapter II, 180 japanese quail (Coturnix coturnix japonica) females, eight weeks old, were used during 5 experimental periods of 21 days. The experimental design was completely randomized, with two diets based on amino acids (total and digestible) at three concentrations each (100, 95 and 90%), totaling six treatments, with six replicates of five birds per experimental unit. The experimental rations were formulated with corn and soybean meal, isoenergetic (2800 kcal ME / kg) and isoprotein (20% PB). At the end of each experimental period, feed intake (g / bird), dozen eggs per bird (% / bird), percentage of eggs / bird / day (%), weight (g) and egg mass Kg / kg of feed / dozen egg), Haugh unit (UH), specific gravity (g / cm3) and yolk pigmentation through the colorimetric range of Roche® and the Minolta® chromatograph. Feed consumption increased with the reduction of AAT concentrations and decreased with the reduction of AAD concentrations. There was a linear reduction in egg production, egg weight and egg mass, and worsening of feed conversion by egg mass with reductions in the concentration of amino acids in the diet. Yolk staining improved linearly with the reduction of amino acid concentrations in the feed. Yolk pigmentation was better for birds fed total amino acid based feeds. The formulation for 100% AAD is better for the productive performance of Japanese quails in the laying phase.