Avaliação da toxicidade aguda e crônica do extrato etanólico bruto da casca da raiz de Dioclea grandiflora Mart. ex Benth

• Ano de defesa: 2015
• Autor(a) principal: Ramalho, Josue do Amaral
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Farmacologia; Programa de Pós-Graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos; UFPB
• Palavras-chave em Português: Dioclea grandiflora; Toxicidade; Anatomopatológico; Toxicity; Pathology; CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/8266

Introduction: Dioclea grandiflora, known as stone Mucunã, acts on the Central Nervous System, diseases of the prostate and kidney stones. Objective: Toxicology Assessment Acute and chronic nonclinical crude hydroalcoholic extract from the bark of Dioclea grandiflora, based on normative Statement 4, 18 June 2014 national health surveillance agency (ANVISA). Methodology: For both methodologies were used Wistar rats, both sexes, with the administration of ethanolic extract of the root bark of Dioclea grandiflora (EEBCRDg), orally. On acute toxicity, a group treated with 2000 mg/kg dose and a control group (forward). After administration, the parameters of behaviour for 30, 60, 90, 120, 180 and 240 minutes feed intake and water daily, weekly and haematological parameters by evolution and biochemists, after 14 days of administration of a single dose. The number of survivors recorded to determine the LD50. For chronic toxicity the animals were treated orally (v) with the doses of 10, 50 and 250 mg/kg of EEBCRDg and a control group, during 90 days. We analyzed the water and feed consumption, temperature, blood glucose, weight evolution, behavioural tests such as, rod and the open route, biochemical and Hematological and pathological. Results: In acute toxicity there was statistically significant increase in the consumption of water and feed for both sexes. Increased albumin of males, females reduced alkaline phosphatase, total protein and globulins. Decreased the number of red blood cells in females. In chronic toxicity an increase in temperature of males and females decreased, decreased blood glucose levels in both sexes, decreased water consumption and feed in males, increase and decrease of water and rations in females, increased and decreased by evolution for both sexes, for the corportamental open field test, increased the body postures when walking in females, increased the lift in both sexes, increased cleaning in females, decreased the amount of faecal cakes in females. For the biochemical parameters there was decrease of glucose and albumin, increased creatinine, AST, ALT, LDH, total protein, albumin and globulin for males, an increase in total protein and immunoglobulin in females. Haematological parameters increased red blood cells, hematocrit, leukocytes and decreased the VCM and MCHC in males, females increased, decreased red blood cells MCV and MCH. Showing inflammatory infiltrate in the lungs of males. Conclusion: According to the results of the EEBCRDg presented a considerable toxicity, and required further investigation to attest to the safety of its use.