Efeito anticonvulsivante e ansiolítico do lipídio do leite caprino enriquecido com ácido linoleico conjugado em camundongos swiss

• Ano de defesa: 2015
• Autor(a) principal: Luna, Vanessa Resende
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Ciências da Nutrição; Programa de Pós-Graduação em Ciências da Nutrição; UFPB
• Palavras-chave em Português: Lipídio; Leite de cabra; Convulsão; Ansiedade; Lipid; Goat milk; Convulsion; Anxiety; CIENCIAS DA SAUDE::NUTRICAO
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/8829

Goat's milk is rich in short-chain fatty acids (caproic, caprylic and capric) and average-chain fatty acids (myristic and lauric acid), besides having Conjugated Linoleic Acid (CLA), which has demonstrated neuroprotective effects. Thus, this study aimed to investigate the anticonvulsant effects of the lipids from goat milk enriched with CLA in Swiss mice. Animals received by gavage 1 ml/100g of fat goat milk (LIP group) or water (CONT group) for 30 days. After supplementation, it was performed maximal electroshock test (MES), the test of induced convulsions by pentilenotrazol (PTZ) and the kindling model. It was also carried out evaluation tests of locomotor (rota-rod and open-field) and anxiolytic (elevated plus maze - EPM) activity and biochemical evaluation of the animals. LIP group demonstrated a significantly lower consumption of feed when compared to the control (CONT 7.4 ± 0.9g, LIP 5.6 ± 0.9g LIP; p <0.01) and had significantly lower weight (CONT 36.3 ± 3.4g; LIP 33.2 ± 3.7g; p = 0.04). There was no statistical difference in water consumption (CONT 8 ± 0.7 mL, LIP 8 ± 1.1mL; p = 0.54). For the PTZ test, LIP group presented latency to onset of convulsions and death significantly higher (233.5 ± 103.4s and 75.4 ± 754s, respectively) when compared to CONT (67.1 ± 24.5s and 459.2 ± 75.5s, respectively), with p = 0.02 and p = 0.03, respectively. For the MES test, LIP group showed a significant reduction in the duration of the seizure (5.1 ± 2.4s) compared to CONT (13.3 ± 3.5s). Both tests have also demonstrated significant effects on the severity of seizures. For kindling mice, despite the LIP group present a latency to onset of convulsions greater (571.6 ± 86.3s) than the CONT (543.7 ± 171.3s), this result was not statistically significant (p = 0.57), as well as the score convulsions (p = 0.12). Fat presented anxiolytic effect in the EPM, because LIP almost doubled the number of entries into the open arms (p = 0.04), in addiction to increase time spent in these arms (CONT 25.1 ± 18.9s, LIP 48 ± 23.62s, p = 0.04) and enhanced the number of dips (p < 0.05). There was no statistical difference between the groups in terms of locomotor activity. In biochemical assessment LIP provided a significant increase in AST (CONT 105.8 ± 26.64; LIP 131 ± 20.31; p < 0.05); amylase (CONT 1061 ± 219.7; LIP 1726 ± 405.3; p < 0.01) and FA (CONT 369.1 ± 94.66; LIP 580.3 ± 154.5; p < 0.01) and a reduction of calcium levels (CONT 7.78 ± 3.27; LIP 4.56 ± 1.58; p < 0.01). We conclude that goat's milk lipids had effect on the central nervous system, with anticonvulsant and anxiolytic activity, without affecting the motor control of the animals.