Suplementação alimentar com Spirulina platensis promove efeito antiobesidade e restaura a reatividade contrátil de íleo em ratos Wistar

• Ano de defesa: 2017
• Autor(a) principal: Ferreira, Elba dos Santos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Farmacologia; Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos; UFPB
• Palavras-chave em Português: Obesidade; Spirulina platensis; Reatividade contrátil e relaxante; Estresse oxidativo; Íleo de rato; Obesity. . .; Contraction and relaxing reactivity; Oxidative stress; Rat ileus; CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/12332

Obesity is a chronic disease characterized by the accumulation of adipose tissue. Spirulina. Platensis is a blue-green algae and has been shown to be a promising drug in potentiating rat aortic reactivity. The objective of this study was to evaluate the effects of obesity, supplementation with S. platensis and dietary changes on nutritional and morphometric parameters, muscle reactivity in ileus and oxidative stress in rats. The animals were divided in six groups: saline control (GCS), obese saline (GOS), obese supplemented with S. platensis at doses of 25, 50 and 100 mg / kg (GO + SP25, GO + SP50 and GO + SP100, Respectively) and obese fed with Presence® diet (GO + DP). The animals received the DP and hypercaloric diets for 16 weeks and the supplementation from the 8th week. GO + DP received the hypercaloric diet for eight weeks and PD for eight weeks. During the 16 weeks, food intake and body mass were evaluated and, after euthanasia, the adipose epididymal, retroperitoneal and inguinal tissues were used to calculate the adiposity index. The ileum was suspended in vats for isolated organs and the isotonic and isometric contractions monitored. The protocols were approved by the Committee on Ethics in the Use of Animals / UFPB (017/2016). Food intake was reduced in GOS, and increased in animals supplemented with S. platensis and in GO + PD in relation to GOS. GOS did not differentiate in relation to GCS regarding initial, mean, final body mass and body mass gain. In the groups supplemented with S. platensis, only the GO + SP25 differentiated as regards mean, final body mass and body mass gain in relation to GOS. The GOS presented the upper CEA and the lower CCA to the GCS. The groups supplemented with S. platensis showed no difference in any of these coefficients in relation to GOS. The GO + DP presented an increase in the CEA and CGPCC and a decrease in the CAA in relation to the GOS. GOS showed an increase in the masses of the three adipose tissues, adiposity index and adipocyte diameter, in relation to GCS. In the S. platensis treated groups, only the 25 mg / kg dose did not alter the adipose tissue mass and the adipocyte diameter in relation to the GOS, however, all doses of S. platensis and GO + DP decreased the index (62.1 ± 6.1%) and CCh (60 ± 6.4%), compared to GCS (100%) and the relaxing potency of verapamil (11.6 ± 0.6) compared to GCS (13.7 ± 0.2). S. platensis (50 mg / kg) reversed the efficacy of KCl (88.3 ± 3.3%) and CCh (86.8 ± 5.4%) relative to GCS. However, S. platensis (100 mg / kg) attenuated the relaxing potency of verapamil over GOS (11.6 ± 0.6 vs 9.0 ± 0.2). The thickness of the circular and longitudinal muscle layers of rat ileum was not altered in GOS, as was the concentration of malondialdehyde (MDA) in plasma and ileum. In the epididymal and retroperitoneal adipose tissues, GOS showed an increase in MDA, and only S. platensis (50 mg / kg) reversed this effect. There was no change in antioxidant activity in plasma, ileus and adipose tissues studied in GOS, however the dose of 50 mg / kg of S. platensis increased CAT in adipose tissue. It is concluded that obesity decreases food intake, contractile and relaxing reactivity of ileum and increases lipid peroxidation in adipose tissue. Supplementation with S. platensis (50 mg / kg) increases food intake, restores the contractile reactivity of ileus, and reverses oxidative stress in adipose tissue. Dietary change induces antiobesity effect, but does not reverse the contractile reactivity of the ileum and oxidative stress in adipose tissue.