Influência do polimorfismo C677T no gene MTHFR relacionado à suplementação de uma dieta com folato no status inflamatório de mulheres obesas e com sobrepeso

• Ano de defesa: 2017
• Autor(a) principal: Lisboa, Jéssica Vanessa de Carvalho
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Ciências da Nutrição; Programa de Pós-Graduação em Ciências da Nutrição; UFPB
• Palavras-chave em Português: Interleucinas; Fator de Necrose Tumoral; Intervenção dietética; Polimorfismo; Folato; Sobrepeso; Obesidade; Interleukins; Tumor Necrosis Factor; Dietary intervention; Polymorphism; Folate; Overweight; Obesity; CNPQ::CIENCIAS DA SAUDE::NUTRICAO
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/12277

Background: The C677T polymorphism of the methylenetetrahydrofolate reductase gene (MTHFR), participates in the metabolism of folic acid, since the gene encodes the MTHFR enzyme that with reduced activity elevates serum levels of homocysteine and can trigger inflammatory process. Therefore, it is necessary a deep research to identify possible relationships between the intake of folate in minimizing these risk factors and the response of obese women to the polymorphism. Objective: the study aimed to evaluate the influence of the C677T polymorphism on the MTHFR gene on the effects of nutritional intervention strategies with folate in the inflammatory process in overweight women. inflammatory process in overweight and obese women. Methods: A double blind intervention study was carried out with 48 obese or overweight adult women, aged 20 to 59 years, selected from a population-based study entitled "II Cycle of Diagnosis and Intervention of the Food, Nutritional and Non-Communicable Diseases of the Population of the Municipality of João Pessoa / PB", focused on the municipality of João Pessoa. Food consumption was evaluated using a 24-hour Dietary Recall (24HR), also made the test of C677T polymorphism, biochemical parameters such as folic acid, vitamin B12, Homocysteine, TNF-α, Interleukins and anthropometric evaluation. The 48 women divided into two groups with 24 participants and sub-stratified into 3 groups according to the CC, CT and TT genotypes and polymorphism genotyping analysis C677T from the MTHFR gene. These two groups received 300g of vegetables daily for 8 weeks, with 191 mcg/day of folate for Group 1 and 95 mcg/day for Group 2, in order to evaluate the effect of the folate contained in this diet on the levels of the inflammatory markers and in what way the C677T Polymorphism would influence in this process. The statistical analysis was performed by assay of variance (ANOVA), Student's t-test, Tukey's test and simple linear regression using Software R, with p-value significance <0.05. significance for p-value <0.05. Results: a significant increase in folate intake and folic acid intake in all genotypes (CC, CT, TT) was observed in group 1 that received dietary intervention containing 191 mcg / d of folate. TNF-α, IL-6, IL-1β were also reduced in G1 but only in the TT genotype. In group 2, with an intervention containing 95 mcg / d of folate, there was a significant difference in folate consumption values for the CC and TT genotypes, folic acid post intervention for the CT and TT genotypes, 13.32 μg / mL ± 3.97 and 15.00 Μg / mL ± 4.60 to 16.4 μg / mL ± 4.95 and 22.62 μg / mL ± 6.30, respectively; Reduction in IL-1β post-intervention values from 5.43 pg / ml to 1.55 pg / ml in the CT genotype. Conclusions: Based on results after dietary intervention with folate being observed elevation of folic acid values, reduction of interleukins, TNF-α, and homocysteine mainly in the TT genotype.