Avaliação de propriedades hematológicas de nanofibras de titânio (TiO2) produzidas pelo método de Solution Blow Spinning (SBS)

• Ano de defesa: 2018
• Autor(a) principal: Cruz, Sara Brito Silva Costa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Odontologia; Programa de Pós-Graduação em Odontologia; UFPB
• Palavras-chave em Português: Nanofibras; Titânio; Hemocompatibilidade; Coagulação; Nanofibers; Titanium; Hemocompatibility; Coagulation; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/19710

Hemorrhages are complications that can occur after trauma or surgical procedures. In the oral cavity, such phenomenon can happen mainly due to the high vascularization present in this region and this fact should lead the dental surgeon to adopt measures that favor good hemostasis. Although several types of hemostatic agents are available on the market, some of them still have limitations of use and disadvantages. In this scenario, the thrombogenic potential and the actions in the coagulation process have been investigated in several materials, among them, titanium nanofibers, which due to their peculiar properties and their biomimetic potential became attractive for numerous applications. Hence, this work aims to evaluate the hematological and thrombogenic properties of three titanium (TiO2) nanofibers produced by Solution Blow Spinning (SBS) process, identified as HemoFiber 1, Hemofiber 2 and HemoFiber 3 and worked in different concentrations.All fibers were characterized using methods such as physical analysis to determine wettability by measuring the contact angle, X-ray diffraction and scanning electron microscopy for surface images. Subsequently, biological tests of cytotoxicity, hemolysis, and haemagglutination were performed. Coagulation time was measured through Lee-White technique when nanofibers were contacted with whole blood samples from healthy volunteers (n = 10). The tested materials showed high hydrophilicity, with zero contact angle and distinct crystalline phases. Good cell viability, haemagglutination characteristics and coagulation time reduction ability were also observed. Cell viability was greater than 100 percent for HemoFiber 1 and 2 and decreased on HemoFiber 3 with increasing concentrations. Hemolysis rates ranged from 0 to 18.52%, with HemoFiber 1 having the highest values. For coagulation times, the data were analyzed from the ANOVA test, considering p <0.05 There was a statistically significant difference between all groups in relation to the control (dry tubes). HemoFiber 3 presented the shortest times (330.18 ± 29.88). The variation of concentrations in the same group was not significant for Hemofibers 1 and 3, while HemoFiber 2 obtained better times when used the highest concentration (C3). Thus, the obtained results suggest that TiO2 nanofibers exhibit hemocompatibility and substantial hemostatic potential, suggesting the use in containing or controlling surgical sites minor hemorrhages. However, it is necessary to perform new studies to better understand the behavior and blood interactions of these nanostructures.