Atividade do coriandrum sativum l. sobre cepas de escherichia coli produtoras de β-lactamases de espectro estendido

• Ano de defesa: 2014
• Autor(a) principal: Pedrosa, Zilmara Vieira
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraí­ba; BR; Farmacologia; Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos; UFPB
• Palavras-chave em Português: E. coli ESBL. C; Sativum; Óleo essencial; Fitoconstituinte; E. coli ESBL; Coriandrum sativum; Essential oil; Phytochemicals; CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/tede/6817

There are some strains of Escherichia coli that are pathogenic and often cause urinary tract infections, septicemia and meningitis in neonates. Some produce enzymes known as β-lactamases of extended spectrum (ESBL) that decrease the therapeutic options, thereby arousing interest in the discovery of new antibacterial products. Medicinal plants and their essential oils are especially rich in metabolites with antimicrobial properties as soon researches are developed in searches of these substances. Thus, the present study aimed to evaluate the effect of essential oil of Coriandrum sativum and its major phytochemicals against strains of E. coli ESBL. Was initially determined the sensitivity of the tested strains to conventional antibiotics, as well as screening of the antibacterial activity of the oils against these strains. Chosen C. sativum oil, its chemical composition was determined by gas chromatography coupled to mass spectrometer (GC/MS) and the antibacterial activity of both the oil as the major phytochemicals were evaluated by determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), the microdilution technique, the kinetics of microbial death oil was also analyzed. It has also played a modulating effect over the conventional antibiotics. It was found that the tested strains are producing ESBL. Among the phytochemicals, linalool (39.78%) presented itself as a major component, followed by linalool oxide (27.33%). The oil and linalool inhibited all strains having MIC and MBC of 256 μg/mL, 512 μg/mL and 1024 μg/mL, between 512 and 1024 μg/mL, respectively. Linalool oxide was inactive at the maximum test concentration of 1024 μg/mL. This oil has antibacterial activity dependent on its concentration and time of exposure in the micro-organism. Both oil and linalool modulates the action of the antibiotics ciprofloxacin and cefoxitin, synergistic interaction of oil and ciprofloxacin being observed. The combination with linalool showed synergistic effects with ciprofloxacin and norfloxacin. The results suggest that the essential oil of C. sativum can suppress the growth of species E. coli ESBL and its major phytochemical despite having weak antibacterial activity, may modulate the action of some antibiotics.