Desenvolvimento e avaliação de espuma dental, contendo óleo essencial de Lippia sidoides Cham. para prevenção e tratamento de lesões bucais

• Ano de defesa: 2019
• Autor(a) principal: Santos, Alessandra Estevam dos
• Tipo de documento: Tese
• Tipo de acesso: Acesso embargado
• Idioma: por
• Instituição de defesa: Universidade Federal da Paraíba; Brasil; Farmacologia; Programa de Pós-Graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos; UFPB
• Palavras-chave em Português: Lippia sidoides Cham; Lesões na cavidade bucal; Óleo essencial; Espuma dental; Lesions in the oral cavity; Essential oil; Dental foam; CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: https://repositorio.ufpb.br/jspui/handle/123456789/20038

Introduction - Ulcerative lesions in the oral cavity cause pain and interfere in the patient's quality of life. Objective - To evaluate in vitro the essential oil of Lippia sidoides Cham., In the perspective of developing a pharmaceutical form indicated for lesions in the buccal cavity. Methods - The essential oil of Lippia sidoides Cham. (OELS) was extracted from the aerial parts of the plant and its oil was characterized by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The major component, thymol, was quantified using a method developed and validated in Gas Chromatography with Flame Ionization Detector (CG-FID). A seasonal study was carried out to verify thymol content and yield in relation to climatic variables. The antimicrobial activity of OELS, the essential oils associated with Cymbopogon citratus and Citrus limon and the developed dental foam was verified against the microorganisms Pseudomonas aeruginosa, Streptococcus mutans, Staphylococcus aureus and Candida albicans through agar diffusion, microdilution and checkerboard assays and cytotoxicity of oils evaluated. A dental foam was developed in which the essential oils were incorporated. Using a freeze-drying method, a microparticle was developed, containing OELS from a factorial design, and a solid matrix extraction method was developed and validated for determination and quantification of thymol in the microparticle. Results - The yield of the OELS and the thymol content are influenced by the maximum temperature and minimum temperature, respectively. Regarding chemical composition, qualitative and quantitative changes occur throughout the year. The study oils presented antimicrobial activity against all the microorganisms tested, and the Minimum Inhibitory Concentration (MIC) was determined for each of them, ranging from 1.45 - 4.72 mg.mL-1 to OELS, 0.46-2 , 94 mg.mL-1 for OECC and 8.48 - 169.69 mg.mL-1 for OECL. The dental foam at all tested concentrations presented antimicrobial activity against the microorganisms of the study, presenting larger halos of inhibition against C. albicans (18.4 - 23.7 mm). In the development of the microparticles, it was verified that the experimental conditions containing hydroxypropylmethylcellulose (HPMC) presented a higher percentage of thymol recovery. Conclusion - The developed dental foam presented antimicrobial activity against all the microorganisms of the study in all concentrations and associations tested. In conjunction with the cytotoxicity tests performed, the results point to a promising formulation for the therapeutic management of oral mucosal ulcerative lesions.