| Resumo: |
Ultraviolet radiation induces several harmful effects on skin exposed to solar radiation. Due to the tropical location, among other factors, skin cancer is very common in Brazil. Another effect caused by excessive sun exposure, in addition to skin cancer, is photoaging, which has the potential to motivate the population to use sunscreens in order to alleviate this unwanted effect. In this sense, Dihydromyricetin (DHM) is a flavonoid that presents several pharmacological properties with emphasis on antioxidant, antitumor and photoprotective activities, among other beneficial biological effects. Another component, with potential application to minimize the effects of solar radiation, grape seed oil (GSO) is a source of vitamin E, contains fatty acids with high antioxidant potential, high amounts of tocopherols and linoleic acid, being known to its effect on increasing the sun protection factor after inclusion in photoprotectors. Therefore, the present work aimed to develop, characterize and evaluate the cytotoxicity of Eudragit RS100® nanocapsules containing the active ingredient DHM and grape seed oil. Firstly, the pre-formulation study was carried out, investigating the possible occurrence of some swelling effect or dissolution of the polymer by the GSO, and soon after, the nanocapsules (NC) were prepared, which were in the nanometric range. The stability of the nanocapsules for 90 days under different storage conditions was carried out and demonstrated that the best conditions for storage, without loss of physical and chemical characteristics, were under refrigeration and room temperature. The pH values of the NC were suitable for skin application. The zeta potential was positive due to the cationic properties of the polymer used as the polymeric wall. The results of the cytotoxicity and genotoxicity of cationic nanocapsules containing DHM in HFF1 and HaCat cells, as well as their interaction with the DNA molecule. The results demonstrate that DHM, when nanoencapsulated, maintained its antioxidant capacity and prevented the cytogenotoxic effect of the cationic polymer, reinforcing that its use can be better explored by nanomedicine for different biological applications. The results of cell viability assays by MTT for nanocapsules without DHM demonstrated a reduction in cell viability. |
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