Detalhes bibliográficos
| Ano de defesa: |
2023 |
| Autor(a) principal: |
Sangoi, Gabriela Geraldo |
| Orientador(a): |
Machado , Alencar Kolinski |
| Banca de defesa: |
Favarin , Fernanda Reis,
Tonel, Mariana Zancan |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Franciscana
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Nanociências
|
| Departamento: |
Biociências e Nanomateriais
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1198
|
Resumo: |
Although the inflammatory response is fundamental for the protection of the body, this mechanism must be controlled. Individuals with neuropsychiatric diseases have chronic inflammation and oxidative stress, the former being activated by the Damage Associated Molecular Patterns (DAMP) pathway and the latter mainly by mitochondrial dysfunction. Furthermore, the pathophysiology of such diseases is not fully elucidated. Thus, searching for new treatment alternatives that address these innovative aspects is of great value. In this context, the present work aims to evaluate the neuroprotective effect of the Euterpe oleracea Mart. extract, açaí, in its free form (EAL) and liposomal form (LEA), against damage caused by quinolinic acid (QA), a neurotoxin, because natural products have been standing out in this context. The use of nanotechnology was thought to improve the bioavailability of the compound and its stability, as well as a strategy to cross the blood-brain barrier. In addition, the LEA was produced, characterized and evaluated your cytotoxic effect, in addition to analyzing the antidepressant potential of EAL and LEA, since by proving the neuroprotective character against QA, which in turn once its levels are increased in the organism through the gene overexpression of the enzymes indoleamine-2,3-dioxygenase (IDO) and kynurenine-3-mono-oxygenase, through the inflammatory process, it is suggested that the clinical signs of patients with depressive disorders end decreasing, as there is a decrease in neurotoxicity. For this, cell culture of microglia was carried out. Such cells were exposed to EAL and LEA concentration-effect curves as well as to an QA curve for standardization of damage at different times. Having decided on the best damage induction time and concentration, the samples were then added and the neuroprotective capacity of EAL and LEA was evaluated. After the treatments, tests were performed to analyze viability and cell proliferation and oxidative metabolism. In short, the genomodifying and hemolytic capacity were also evaluated, where they presented a genoprotective and non-hemolytic profile. As for the characterization, the EAL presented catechin as the major compound and the LEA obtained indices consistent with nanometric systems and better thermal stability from 1ºC to 7ºC. In addition, the neuroprotective effect of both against AQ was controlled, which provides their use for the alternative treatment of neuropsychiatric diseases, since they protect the central nervous system from the neurotoxicity established by the increase in the AQ regimen in individuals with such chronic diseases. |
