DETERMINAÇÃO DE LIPOPOLISSACARÍDEO EM NANOTUBOS DE CARBONO NÃO FUNCIONALIZADOS E FUNCIONALIZADOS

• Ano de defesa: 2011
• Autor(a) principal: Martins, Sandro Goulart
• Orientador(a): Rodrigues Junior, Luiz Carlos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Franciscana
• Programa de Pós-Graduação: Mestrado Acadêmico em Nanociências
• Departamento: Biociências e Nanomateriais
• País: BR
• Palavras-chave em Português: Nanotubos de Carbono; Lipopolissacarídeo; Viabilidade; Proliferação; Esterilização
• Palavras-chave em Inglês: Carbon Nanotubes; Lipopolysaccharide; Viability; Proliferation; Sterilization
• Área do conhecimento CNPq: CNPQ::ENGENHARIAS
• Link de acesso: http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/263; http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/349

The Carbon Nanotube (CNT) are cylindrical structures of carbon with chemical and physical properties that make them potentially useful in mechanical applications and electrical microscopic scale. Its name is on the feature to its diameter, being hundreds of times thinner than a human hair and more invisible for the most powerful electron microscopes. Contamination of nanomaterials for therapeutic application-diagnosis can occur at any stage of the process. One of the major contaminants is lipopolysaccharide (LPS). Once infected removal is difficult, since the LPS are resistant to high temperatures and pH extremes. The objective of this work is to determine LPS in Wall Carbon Nanotube Multi (NTCPM) and Carbon Nanotubes Wall Multiple carboxyl (NTCPM-COOH). For testing in vitro, splenocytes were isolated from BALB/c mice. The detection of LPS was performed using the method of gel in tube, Limulus Amebocyte Lysate (LAL) gelation method and plate with a sensitivity of 1UE/mL, the NTC were sterilized in an autoclave, oven and autoclave Pasteur in three cycles. LPS was detected in the sample that was not sterile and has suffered only one autoclave cycle, already formed and autoclaved at 3 cycles was removed all the LPS. Cell viability and proliferation test were performed in sterilized and unsterilized samples at a concentration of 10ng/mL. The results showed no significant difference (p <0.05) in the test cell viability of splenocytes, although it was observed a slight decrease in the concentration of LPS with sterile materials, since the LPS decreases cell proliferation and a increased frequency of cells directly proportional to the concentrations of suspensions NTCPM time of 48 hours.