"Draft" genoma e análise filogenética do Trypanosoma caninum

• Ano de defesa: 2020
• Autor(a) principal: Moreira, Janaina Marcela Assunção Rosa
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Agronomia, Medicina Veterinária e Zootecnia (FAMEVZ); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Ciências Veterinárias
• Palavras-chave em Português: Cão; Filogenia; Sequenciamento de nova geração; Trypanosoma caninum; Tripanosomatídeo; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA; Dog; Next generation; Sequencing phylogeny; Trypanosomatid
• Link de acesso: http://ri.ufmt.br/handle/1/4524

Described in the last decade, infecting dogs in several Brazilian regions, biological and epidemiological aspects of Trypanosoma caninum infection are still unknown. Accept advances in New Generation Sequencing (SNG) techniques, the study of an evolutionary biology of trypanosomatids that had a substantial increase in genetic information. Thus, the objective of this study was to present information about the genome of this species and to carry out a genetic comparison with other trypanosomatids, using the Miseq platform for partial genome sequencing. Phylogenetically, T. caninum demonstrated proximity to T. cruzi and T. rangeli. The adaptation of this parasite to the domestic dog and a probable reduction in its genome may be related to its evolutionary process. In addition, genome sequencing also made it possible to investigate target genes / proteins for their specific diagnosis. Thus, the 5 '-AGCCAATAGCAAGGGTGGAA-3' and 5 '- GCGTCATCCATTTTCGAGGC-3' oligonucleotides were designed in the 18S region of the T. caninum rDNA that amplifies a 248 bp product. These oligonucleotides are the most sensitive and sensitive to T. caninum, but the minimum detection limit was 10 DNA records of the parasite.