Ocorrência de genes de citotoxinas, toxinas esfoliativas e pirogênicas em isolados de Staphylococcus pseudintermedius de espécies domésticas e silvestres

• Ano de defesa: 2016
• Autor(a) principal: Pitchenin, Letícia Camara
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Agronomia, Medicina Veterinária e Zootecnia (FAMEVZ); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Ciências Veterinárias
• Palavras-chave em Português: Enterotoxinas; Fatores de virulência; Hemolisinas; Leucocidinas; Síndrome do choque tóxico; Staphylococcus intermedius; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA; Enterotoxin; Hemolysin; Leucocidins; Toxic shock syndrome; Virulence factors
• Link de acesso: http://ri.ufmt.br/handle/1/1163

Staphylococcus pseudintermedius is a coagulase-positive species of SIG (Staphylococcus intermedius Group). It is an opportunistic pathogen that can cause infections in several places and also has zoonotic potential. Although knowledge about the pathogenic process of S. pseudintermedius is limited, it is known the arrest of several virulence factors of this pathogen. Toxins are included in these factors, and epidemiological studies of their distribution in S. pseudintermedius are virtually nonexistent. They can be classified into three main groups: pyrogenic toxin superantigens with properties (SEs and TSST-1), exfoliative toxins and cytotoxins (leucocidins, hemolysin). In this study the occurrence of toxins 8 (sea, sec, tst-1, SIET, EXI, LuK F-I, Luk S-I and hlg ƴ) was studied in isolates of S. pseudintermedius by Polymerase Chain Reaction in 4 species of domestic animals (dogs , cats, cow and swine) and two wild species (Crab-eating Fox and Crab-eating Raccoon) stemmed from several sites of infection. All samples showed the gene of at least one type of toxin, alone or in combination. The highest occurrences were relates in LukS-I (94.83%), Luk F-I (91.38%) and EXI (91.38%) and the lowest in hlg ƴ (5.17%). There was association of occurrence of hlg ƴ and LukF-I genes (p = 0.0175). The extensive occurrence of these genes indicates an important role in the severity of infections caused by these pathogenic commensal microorganisms when