Efeito da restrição proteica durante a prenhez sobre a produção do Peptídeo semelhante ao Glucagon – 1 (GLP-1) em ilhotas pancreáticas

• Ano de defesa: 2019
• Autor(a) principal: Silva, Gleicielly Leite Vieira da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Nutrição (FANUT); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Nutrição, Alimentos e Metabolismo
• Palavras-chave em Português: GLP-1; Convertases de pró-hormônio; Célula alfa; Restrição proteica; Gestação; Rato; CNPQ::CIENCIAS DA SAUDE::NUTRICAO; Prohormone convertases; α-cell; β-cell; Protein restriction; Pregnancy; Rat
• Link de acesso: http://ri.ufmt.br/handle/1/3150

We evaluated whether protein restriction during pregnancy alters intra-islet GLP- 1 production. Control non-pregnant (CNP) and control pregnant (CP) rats were fed a 17% protein diet, and rats in the low-protein non-pregnant (LPNP) and low- protein pregnant (LPP) groups were fed a 6% protein diet; all rats were evaluated at 20 d of pregnancy. The glucose tolerance curve showed that glycaemia at baseline and at 30 and 120 min after glucose load, as well the total area under the glucose curve, was lower in pregnant rats than in non-pregnant rats. Insulin secretion in response to 16.7 mmol/L glucose was lower in islets from the LPP group than in those from the other groups. PC2 and GLP-1 fluorescence in α- cells was lower in the low-protein groups than in the control groups. The least PC2/glucagon colocalization was observed in the LPP group, with the most in the CP group. There was less PC1/3/glucagon colocalization in the LPNP group than in the CNP group and in the LPP group than in the CP group. GLP-1/glucagon colocalization was similar in the LPP, CP and CNP groups, which showed less GLP-1/glucagon colocalization than the LPNP group. There was more Ki67/glucagon colocalization in the LPP group than in the LPNP, CP and CNP groups. PC1/3 and proglucagon mRNA expression levels were higher in the pregnant groups than in the non-pregnant groups. PC2 transcript levels were higher in the low-protein groups than in the control groups and higher in pregnant rats than in non-pregnant rats. These results indicate that protein restriction during pregnancy impairs intra-islet GLP-1 production. This effect may contribute, at least in part, to reduced insulin secretion.