Avaliação do balanço redox e do estresse oxidativo em fígado de ratos recuperados da desnutrição intrauterina e na lactação com dieta à base de farinha de soja

• Ano de defesa: 2012
• Autor(a) principal: Oliveira, Ilkilene Taques Camargo
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Nutrição (FANUT); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Nutrição, Alimentos e Metabolismo
• Palavras-chave em Português: Estresse oxidativo; Antioxidante; Recuperação nutricional; Fígado; Rato; Soja; CNPQ::CIENCIAS DA SAUDE::NUTRICAO; Oxidative stress; Antioxidants; Nutritional recovery; Liver; Rats; Soybean
• Link de acesso: http://ri.ufmt.br/handle/1/1783

Objective: We investigated whether nutritional recovery on a soybean flour diet alters liver antioxidant defense systems. Methods: Male rats from mothers that were fed either 17% or 6% protein during pregnancy and lactation were maintained on a 17% casein (CC, LC), 17% soybean flour (CS, LS) or 6% casein (LL) diet after weaning. Assays for gene expression, antioxidant enzyme activity, protein and lipid oxidation markers, and nitric oxide were performed, and the concentrations of hepatic enzymes, serum transaminases and catecholamine were determined. Results: The CS and LS rats had lower final body weights than the CC and LC rats, but the body weights of the LS and LC groups were higher than those of the LL group. Catalase mRNA was higher in the LS and CS groups than in the LC and CC groups. The LS group had higher catalase mRNA levels than the LC group, and the LC group levels were higher than the LL group. The LS and LC rats had higher glutathioneperoxidase mRNA levels than the LL rats. Liver SOD activity was lower in the LS and LC groups than in the CS and CC groups, as well as in the LS and CS groups than in the LC and CC groups. In the LS and CS rats, liver CAT activity was lower compared to the LC and CC rats. The thiol non-protein content was lower in the LS and CS rats than in the LC and CC rats. Protein and lipid oxidation markers and nitric oxide concentrations were similar in the LC and LS rats, and both were lower than in the LL rats. The CS group had higher serum alanine aminotransferase concentrations than the CC and LS groups. Conclusion: A soybean diet suppressed the hepatic antioxidant defense capacity and apparently compromised the hepatic function in offspring that were fed a soybean diet after weaning following weaning after they were born to and suckled by mothers that were fed a control diet. However, soybean (LS) was as efficient as casein (LC) for recovering weight gain and antioxidant status in the liver, without alterations in stress parameters or signs of toxicity.