Análise proteômica do fungo Conidiobolus lamprauges cultivado em diferentes temperaturas

• Ano de defesa: 2013
• Autor(a) principal: Godoy, Isabela de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Mato Grosso; Brasil; Faculdade de Agronomia, Medicina Veterinária e Zootecnia (FAMEVZ); UFMT CUC - Cuiabá; Programa de Pós-Graduação em Ciências Veterinárias
• Palavras-chave em Português: Temperatura de crescimento; Conidiobolus spp; Ovinos; Análise proteômica diferencial; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA; Growth temperature; Sheep; Differential proteome analysis.
• Link de acesso: http://ri.ufmt.br/handle/1/1128

Temperature is one the most important abiotic factors for the development of biologic cicle of entomopathogeni fungus. The pathogenic fungus has developed molecular mechanisms like protein expression related to growth at high temperature to survive at body temperature of the host. Thus, the aim of this study is to analyse the effect of temperature on in vitro growth of the C. lamprauges and to compare the pathogen cultivated at two different temperatures (28oC and 37oC) by proteomic analysis. Five isolated of C. lamprauges from sick sheep were used to analyse the growth at different temperatures. It was inoculated spore suspension at petri dish with 2% Dextrose Sabouraud Agar, they were incubated at 20, 25, 30, 35 e 40oC and the radial growth was measured daily. For the proteomic analysis, C. lamprauges was cultivated at 28oC and 37oC, it was performed the protein extraction and the two- dimensional polyacrylamide gel. The experiment showed that the isolated grown to approximately 35oC and before, stop the growth. There was inhibition of growth at 18 and 42°C. After differential proteome analysis, a total of 51 and 49 spots were found at 28 and 37oC, respectively, 16 spots were differentially expressed, seven (7/16) were down expressed and nine (9/16) were over expressed at 37oC compared with 28oC.