ABORDAGEM METALOPROTEÔMICA DO MERCÚRIO EM AMOSTRA DE LEITE MATERNO NAS COMUNIDADES RIBEIRINHAS DA REGIÃO AMAZÔNICA - BRASIL

• Ano de defesa: 2022
• Autor(a) principal: Rafael Rodrigo Piva Vasconcelos
• Orientador(a): Felipe André dos Santos
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Fundação Universidade Federal de Mato Grosso do Sul
• País: Brasil
• Palavras-chave em Português: Mercúrio; leite materno; metaloproteínas; biomarcadores; eletroforese bidimensional; espectrometria de absorção atômica; espectrometria por eletrospray acoplada a massas.
• Link de acesso: https://repositorio.ufms.br/handle/123456789/4483

Given a problematic characterization of differentiation through natural sources and identified by natural sources of identification, in these possible works, linked to, an indication of natural protein markers by this metal in lactating women. Twenty-three samples of milk and hair were collected from riverine lactating women from the communities of Livramento-AM – Rio Negro and Abunã-RO, Fortaleza de Abunã-RO, Nova Mutum Paraná-RO and Vila da Penha-RO – Rio Madeira. The quantification of total mercury in the milk samples was performed by graphite furnace atomic absorption spectrometry (GFAAS) and the results were between 6.03 ± 0.48 and 83.49 ± 1.08 µg L-1. As for the total reference concentration in the hair samples, the absorption technique was performed using the optics dedicated to continuous samples – Perkin Elmer's SMS 100, and the results were between 0.33 ± 0.005 and 6.04 ng g-1. The proteome fractionation process was performed by two-dimensional electrophoresis - 2D PAGE, with two replications per sample. The number of separate protein spots was between 92 ± 2 and 347 ± 3. The apparently average between the gels was between 75.5 and 94.5%. As points in relation to isoelectric points and molecular mass, a distributed distribution of distributors9 (isoelectric point) between 15 to 7kDa (molecular mass). The mercury in the spots performed by GFAAS indicated mapping in the range of 0.011 and 0.0158 ng/mL. The points associated with mercury were characterized by sequence mass spectrometry coupled with liquid chromatography - LS-MS/MS. Beta-casein O was identified in 35% of the spots, 35% the constant kappa immunoglobulin OS, 26% the alpha-S1-casein OS and 4% the immunoglobulin chain. These proteins were in contact with the most expressed, and may be possible biomarkers of breast milk.