Análise descritiva histológica e densidade volumétrica estrutural do tecido renal e esplênico em Boana raniceps (Anura: Hylidae)

• Ano de defesa: 2022
• Autor(a) principal: BRENDA DE OLIVEIRA MARTINS
• Orientador(a): Carlos Eurico dos Santos Fernandes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Fundação Universidade Federal de Mato Grosso do Sul
• País: Brasil
• Palavras-chave em Português: histologia, anuros, rim, baço.
• Link de acesso: https://repositorio.ufms.br/handle/123456789/5110

Anurans are important environmental bioindicators because they depend on aquatic and terrestrial environments. However, few studies have described and evaluated the structure of organs that make up the hematopoietic system. Therefore, this study aimed to describe and characterize the histology of the splenic and renal tissue of Boana raniceps. Seventeen males of Boana raniceps collected at the Base de Estudos do Pantanal (BEP-UFMS) between October and March 2018/2019 were analyzed. Spleen and liver were weighed and processed by histological technique. The splenosomatic and renosomatic indexes were calculated. Tissue sections were stained with Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS), Masson Trichrome (TM), Reticulin (Gomori method), and May-Grünwald- Giemsa-Wright (MGGW). In addition, the structural volumetric density of both organs was evaluated. The mean weight of the individuals was 11.4 ±1.5g, and the mean size (CRC) was 5.9 ±0.3 cm. The IRS represented 0.42% and IES around 0.14% of the individual's weight. The most predominant structures in the kidneys were proximal convoluted tubules (TCP) and distal convoluted tubules (DCT), with 60.4 and 16.8%, respectively. In the spleen, the most predominant structure was red pulp with 79%, followed by white pulp with 12.2%. The SRI of B. raniceps showed a negative correlation with the individual's weight, indicating that renal development stabilizes during adulthood. However, the positive relationship between body weight and CRC indicates that the animal continues to grow and gain weight even in adulthood. The negative correlation between TCP and TCD evidences the function of maintaining water balance in the terrestrial environment since TCP is the structure responsible for absorbing water from the glomerular filtrate. Therefore, it is concluded that descriptive histological studies help obtain knowledge about the splenic and renal tissue of B. raniceps.