Detalhes bibliográficos
| Ano de defesa: |
2021 |
| Autor(a) principal: |
MARIAH OJEDA |
| Orientador(a): |
Renata Trentin Perdomo |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Fundação Universidade Federal de Mato Grosso do Sul
|
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Link de acesso: |
https://repositorio.ufms.br/handle/123456789/3855
|
Resumo: |
Introduction: Breast cancer affects millions of women worldwide and is the second type of cancer that most affects women (behind non-melanoma skin cancer). It is estimated that more than 600 thousand new cases of cancer will occur annually between 2020 until 2022. The use of polytherapy is common in the treatment of cancer and has the advantage of greater efficacy in the different subpopulations of the tumor, in addition to avoiding drug resistance. The association of standardized chemotherapy with natural products such as isoflavonoids and terpenes is of great importance, considering the biological potential in their derivatives. Objectives: To evaluate the action at the cellular level of cytotoxic compounds from Brazilian biodiversity (black propolis and C. Mellifluum) combined with chemotherapeutic agents doxorubicin in the Methodology and Results: The results of cytotoxicity in 2D model in the neoplastic lineage MCF-7 show an IC50 of 4.089µg/mL for SBP (isoflavonoid from black propolis) and 3.895µg/mL for SCM (triterpene originated from C. Mellifluum). The cytotoxicity of these compounds was evaluated after mimicking hepatic metabolism in vitro, observing that there was no inactivation of the substance and the cytotoxic activity was preserved. Both compounds were able to inhibit cell migration in potentially metastatic language, in addition to inhibiting the formation of colonies of neoplastic cells when associated with doxorubicin. The samples were able to stimulate cell membrane damage represented by the elevation of TBARS levels and the response of antioxidant defenses. In the investigation carried out in 3D model, it is noticed that the SCM compound when associated with doxorubicin can penetrate more into the formed spheroid. SBP and SCM were able to promote damage to the cell membrane and interrupt the cell cycle in the G1 phase, before the cell entered the S phase related to the synthesis of genetic material. Conclusions: The data found from the evaluation of the isolated compounds corroborate with that described in the literature regarding cytotoxic activity. It is necessary to advance the studies to better understand the mechanism of inhibition of cell migration and how the compounds act in the cell cycle. Keywords: Combretum mellifluum, black propolis, cytotoxicity, spheroids, cell migration, cell culture |
