Validação de ensaios laboratoriais quantitativos automatizados de dosagem do peptídeo natriurético tipo B e a fração amino terminal

• Ano de defesa: 2009
• Autor(a) principal: Debora Maria Tavares de Andrade
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Validação; Armazenamento; Estabilidade; Peptídeos natriuréticos; Coeficiente de variação; Estatística; Técnicas e procedimentos de laboratório; Peptídeos natriuréticos/administração & dosagem; Estudos de validação; Insuficiência cardíaca
• Link de acesso: http://hdl.handle.net/1843/ECJS-86QNRP

Background: The measurement of endogenous B-type natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) are largely used nowadays in diagnosis, prognosis, and treatment of heart failure. Nevertheless, insufficient data in literature related to assays performances and long-term stability on different storage conditions of natriuretic peptides are available. Methods: For validation data, following modified NCCLS EP15-A protocol, we calculated coefficients of variation within-run (CVI), total (CVT) and recovery index percentage of endogenous natriuretic peptides from triplicates of frozen and refrigerate samples from categorized NYHA patients, in three sequential days using BNP MEIA® and NT-proBNP QIA® . Results: Considering BNP-MEIA® assay, the percentage of average CVI ranged from 6.89 to 16.28 and CVT from 13.22 to 32.35 for frozen samples. For refrigerate samples, CVI ranged from 9.78 to 13.67 and CVT from 13.75 to 50.76. The recovery index percentage, in both storage conditions, ranged from 0.35 to 0.98. For NT-proBNP-QIA®assay, CVI ranged from 1.19 to 2.47 and CVTfrom 2.28 to 5.67 for frozen samples. For refrigerate samples, CVI ranged from 0.55 to 0.78 and CVT from 0.9 to 1.76. The recovery index percentage was nearest 1 in both storage conditions. Conclusions: Our data showed recovery index percentage statistically lower from BNP, in refrigerate and frozen samples, than NT-proBNP, in a time of 48 hours. In addition, coefficient of variation within-run (CVI) of BNP-MEIA® assayin refrigerate and frozen plasma, were statistically highest from values observed in NT-proBNP-QIA® assay. These data must be considered when using these assays to evaluate patient categorization and treatment.