Desenvolvimento e padronização de teste imunoenzimático para pesquisa de anticorpos anti-parvovírus suíno

• Ano de defesa: 1995
• Autor(a) principal: Anapolino Macedo de Oliveira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: suíno; anticorpos; parvovírus; ELISA; Suino Doenças; Parvovirus; Imunoglobulinas; Técnicas imunoenzimáticas
• Link de acesso: http://hdl.handle.net/1843/BUOS-8PKMCU

An indirect enzime-linked immunosorbent assay (ELISA) was standardized for the detection of antibodies to swine parvovirus. The antigens were produced in cultures of primary and continuous cell line (SK6) of swine kidney, and processed by centrifugation (80.000 x g, 4°C, 18 h) through a 30% (v/v) sucrose bed. One hundred and four pig serum samples were used for ELISA standarlization; among them, fifty-one were collected in herds which ones did not use vaccine against swine parvovirus and without any history of this disease. The other fifty-three were collected in herds which used vaccine against swine parvovirus in their preventive programs. The results obtained from ELISA and hemaglutination inhibition test (HI) were sensitive to detect antibodies to swine parvovirus. A strong correlation (r = 0,93) was found between individuals values of absorbance obtained from ELISA test and HI titers. lt was concluded that ELISA test was faster and economic; besides it can be completely automatized.