Efeito do exercício prévio em esteira sobre os níveis de fator neurotrófico derivado do cérebro no sistema nervoso central e soro de ratos submetidos ou não a estresse agudo por contenção

• Ano de defesa: 2011
• Autor(a) principal: Isis Tacyana Goncalves Soares Nascimento
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Neurociências; Exercícios físicos; Exercício em esteira; Fator neurotrófico derivado do cérebro; Stress
• Link de acesso: http://hdl.handle.net/1843/BUOS-8RSMKE

This study aims to evaluate the effect of treadmill on the expression of BDNF in the hippocampus, cerebellum and serum from young and adult rats submitted to acute restraint stress. Twenty-nine young (27 days) and twenty-five adult (180 days) male Wistar rats were used in this study. Moderate-intensity treadmill running was performed daily for 30 min over three weeks. Their running speed was determined according to their individual running performance during the evaluation, corresponding to 5060% of the workload to achieve moderate-intensity forced exercise. Control animals were submitted to individual running performance but not to treadmill exercise, staying in their cages over the period of treadmill running. Control and exercised animals were submitted to acute restraint stress for 120 minutes and then killed immediately or 60 minutes after the restraint stress. The levels of BDNF in the hippocampus, cerebellum and serum were measured by ELISA (mean ± SD, pg / mg total protein for tissue and pg / mL for serum). Our results show that in both young and adult rats the 3-week period of treadmill running did not change the expression of BDNF in the hippocampus, cerebellum and serum. On the other hand, both young and adult rats submitted to restraint stress exhibited lower BDNF levels in the hippocampus, independently on previous treadmill running. However, young rats exhibited a greater resistance to acute restraint stress , since the BDNF levels were reduced only after 60 minutes. In this way, the adult rats showed significant reduction in the hippocampus BDNF levels immediately after restraint stress. Concerning the cerebellum, there was no difference among all the experimental groups, i.e., the acute restraint stress was not able to change the BDNF levels in this organ. Concerning the serum levels of BDNF, the rats submitted to previous treadmill running showed higher values than the control ones 60 minutes after the restraint stress.