Produção de conjugados para identificação de Clostridium septicum e Clostridium chauvoei para a técnica de imunofluorescência direta

• Ano de defesa: 1992
• Autor(a) principal: Maristela Pimentel Pinto
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Clostridium sepcticum; conjugados; Clostridium chauvoei; Imunofluorescência direta; Clostridium; Imunofluorescência
• Link de acesso: http://hdl.handle.net/1843/BUOS-8QLMQR

The production of conjugates anti-Clostridium septicum and anti-Clostridium chauvoei for direct immunoiluorescense testing is described. The culture medium used, enriched with glucose 0,5% and cysteine 0,1%, propitiated fast and expressive C. septibum and C. chauuoefs growing. Antigens containing 3,2x10 8 to 3,4x10 8 cells/ml were considered satisfactory for immune sera production and aglutination tests. Conjugates prepared from immune sera fractionated by combination of caprylic acid and ammonium sulfate precipitation exhibited eight times higher titres than conjugates prepared by ammonium sulfate precipitation. Heterologous antigens and field materials sent to UFMG's Veterinary School were tested. Fluorescent inibition tests and heterologous and homologous antigens adsorption tests were peiformed.