Estrôncio nanocarreado na remissão de quadro semelhante à osteoporose em Zebrafish (Danio rerio)

• Ano de defesa: 2021
• Autor(a) principal: Letícia Cristina Dias Lima
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; Brasil; ICB - DEPARTAMENTO DE MORFOLOGIA; Programa de Pós-Graduação em Biologia Celular; UFMG
• Palavras-chave em Português: Osteoporose induzida por glicocorticoide; Zebrafish; Nanomaterial fosfatado; Ranelato de estrôncio; Biologia celular; Osteoporose; Nanoestruturas; Estrôncio; Peixe-zebra
• Link de acesso: http://hdl.handle.net/1843/35836

Osteoporosis is an osteometabolic disease that affects the mainly postmenopausal women and the elderly, being related to imbalance between the rate of resorption and bone synthesis. The therapies available for clinical treatment use low-absorption drugs that require daily administration or a weekly, monthly, or semiannual treatment with high-cost compounds. Know that the development of more accessible formulations and therapies, helps to obtain a better quality of life for the patient, we present in this dissertation the results of the synthesis and in vivo evaluation of phosphate nanomaterial containing strontium (NPM-Sr), in the treatment of a condition similar to prednisone-induced osteoporosis, having as an animal model zebrafish (Danio rerio) in a period of 7 days, in addition to making a comparison of this treatment, against the strontium ranelate. In this approach we used 4 experimental groups (control - CT, osteoporotic - PN, osteoporotic treated with phosphate nanomaterial containing strontium - NM-Sr and osteoporotic treated with strontium ranelate - R-Sr), composed of 10 animals each. The data were acquired through histological (histochemical and cytochemical) and analytical (EDXMA, TXRF, radiomarking with 99mTc/MDP and ICP-MS) techniques, to value calcium homeostasis, bone remodeling mechanisms and chemical element dynamics. The results show that the nanocarrier strontium can improve the appearance of scales compared to the PN group (mean difference -0.3551, p < 0,05) and serum concentrations of chemical elements such as calcium and copper front this same group (mean difference -0.1697 and -0.0618, p < 0,05, respectively), besides demonstrating less change in strontium concentrations in relation to the R-Sr group (mean difference -0.6414, p < 0,05). These data provide a perspective for the use of this nanomaterial in the treatment of prednisone-induced osteoporosis in Zebrafish, although at concentrations 228 times lower than recommended in the treatment with strontium ranelate.