Produção e caracterização de anticorpos monoclonais anti épsilon toxina de Clostridiun perfrigens tipo D

• Ano de defesa: 2006
• Autor(a) principal: Theonys Diogenes Freitas
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Épsilon toxina; Anticorpos monoclonais; Clostridium perfringens; Clostridium perfringens Toxinas
• Link de acesso: http://hdl.handle.net/1843/SSLA-7VGJ5D

Monoclonal antibodies reactive against épsilon toxin from Clostridium perfringens type D were produced. Myeloma cell lines Sp2/0. Ag14 and P3-X63-Ag8.653 were fused with spleen cells from mice BALB/c immunized with epsilon toxoid. After fusion 59 wells were positive for the Sp2/0. Ag14 cell line and 19 for the P3-X63-Ag8.653, representing 6.68 and 6.59% respectively. Six hybrids were established secreting MoAb of the IgM (k) class and IgG3 (k) subclass. An average of 21.52 g/ml of IgM and 15 g/ml of IgG3 anti-epsilon toxin MoAb were obtained from the hybrids culture supernatant