Ensaio clínico-terapêutico na leishmaniose visceral canina.: Imunoquimioterapia em animais assintomáticos

Detalhes bibliográficos
Ano de defesa: 1998
Autor(a) principal: Marcia Almeida de Melo
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
UFMG
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/1843/BUOS-8QFLSJ
Resumo: The available drugs for treatment of human visceral leishmaniasis do not promote cure of the dogs Whereas new drugs are not available for use in dogs, it is necessary to test therapeutic trials using old drugs in association with other agents. The objective of this experiment was to test the therapeutic efficacy of the Glucantime® in association with an antigenic extract from Leishmania braziliensis in assymptomatic dogs expenmentally infected. Thirty-two laboratory reared mongrel dogs were infected with amstigotes of L. chagasi. Dunng 450 days, the follow up was done by specific antibody detection, cellular immune response, hematology, biochemical serology and detection of the parasite in boné marrow and skin. Alter the confirmation of the infection by antibody or parasite detection, the dogs were randomly allocated in four groups of eight animals each; Group A, received antigen (500 hg/day); Group B, received Glucantime® (100 mg/Kg/day); Group C, received the antigen plus Glucantime® and group D received no treatment. All dogs were killed and necropsied 450 days after inoculation. Bone marrow aspirate, spleen, popliteal lymph node, skin from ear were collected and stained with giemsa for microscopic examination. Tissue samples of skin from nose, ear, mesenteric and popliteal lymph nodes, kidney, spleen and liver , were processed by conventional method and stained with H & E. Spleen samples were inoculated into culture tubes containing NNN/LIT. Thirteen dogs were infeued. The antimonial was able to reduce the antibody levels and the parasitism but the dogs were not cured.