Expressão de ativina A em células-tronco embrionárias de camundongo durante o processo de diferenciação em osteoblastos

• Ano de defesa: 2013
• Autor(a) principal: Bruno Muzzi Camargos
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Proteína morfogenética óssea 4 (BMP-4); Osteoporose; Células-tronco embrionárias; Osteoblasto; Ativina; Osteocalcina; Osteoblastos; Células tronco embrionárias; Proteína morfogenética óssea 4; Ativinas/análise; Diferenciação celular/genética; Camundongos
• Link de acesso: http://hdl.handle.net/1843/BUOS-9JNHG7

The search for anabolic agents has been widely researched for osteoporosis therapy. Embryonic stem cells (ESC), differentiated into osteoblasts, have been used for the study of cell physiology. Protocols osteoblastic cell differentiation have been successfully performed. A common stimulus used on most osteoblastic differentiation protocols is bone morphogenetic protein (BMP) . Activin A, a growth factor with structure and function homologous to BMP, has important effects on the proliferation of osteoblasts and bone remodeling. However, the definition of the exact moment when activin A acts on bone mineralization demand studies. OBJECTIVE: To evaluate the expression of activin A on a mouse ESC protocol used to differentiate them into osteoblasts. MATERIAL AND METHODS: Adopt a technical modification to an osteoblastic ESC culture differentiation protocol (Kawaguchi, 2006), isolated at the Laboratory of Human Reproduction Prof. Aroldo Fernando Camargos - Hospital das Clínicas / UFMG. Real time polymerase chain reaction (PCR) evaluated genic expression at different stages during the protocol for markers of celular indifferentiation (Oct -4, Nanog), markers of osteoblastic differentiation (osteocalcin, bone alkaline phosphatase, RUNX2) and Activin A at different stages of differentiation protocol. RESULTS: Markers of osteoblast differentiation increased as the culture moved towards osteoblastic differentiation. Activin A expression showed a direct association to the markers of bone formation. The protocol favored the differentiation of osteoblasts precursor cells. CONCLUSION: Activin A was expressed in cell cultures of mice ESC since an intermediate stage of differentiation into osteoblasts, following the pattern of cellular expression observed for other markers of bone formation, such as osteocalcin, during the differentiation protocol.