Caracterização e análise das vias de redução de nitrato em Corynebacterium peudotuberculosis biovar equi

• Ano de defesa: 2014
• Autor(a) principal: Carlos Augusto Almeida Diniz
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Corynebacterium pseudotuberculosis; Redução de nitrato; Respiração; Genômica comparativa; Adaptação; Nitratos; Genômica; Genética
• Link de acesso: http://hdl.handle.net/1843/BUBD-9W8H6F

Technologies for sequencing new generation have the potential to decode the genomic diversity at a moderate cost. Corynebacterium pseudotuberculosis is a facultative intracellular parasite, responsible for causing chronic infect-contagious illnesses. The disease presents itself in different ways, in accordance with the affected host. Lineages of C. pseudotuberculosis show high adaptability to different ecological niches and are able to grow in some carbon sources, besides the fact that some specific lineages are capable of achieving the nitrate reduction. The objective of this study is to analyze and propose the route of nitrite reduction of Corynebacterium pseudotuberculosis biovar Equi and to identify the enzymes that constitute the electron transport chain, through analysis in silico. A total of 15 complete genomes of C. pseudotuberculosis were analyzed, in order to identify the respiratory chain and the route of nitrate reduction in silico. Constituent genes of these routes have been identified. Experimental validation was performed through phenotyping and PCR. With this yet unpublished work for the species, comparative genomics and experimental validation are intended to be used, in order to try to understand and characterize metabolic routes associated with breathing still not known in C. pseudotuberculosis. To adapt to a wide range of growing conditions and environmental stress, bacteria have modular respiratory systems that allow them to improve energy production in variable environments, which can be hostile. The respiratory system composition is adjusted according to specific conditions in which they find themselves, by means of a complex network of regulation that allow them to survive and thrive in a particular environment, such as fagossomics intracellular compartments of human macrophages. A greater understanding of the respiratory metabolism of C. pseudotuberculosis will be of great importance to perceive the interactions between pathogen and host and for the development of therapeutic strategies in the future.