Avaliação de vacinas contra Clostridium perfringens tipos C e D

• Ano de defesa: 1997
• Autor(a) principal: Edisio Oliveira de Azevedo
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Minas Gerais; UFMG
• Palavras-chave em Português: Toxinas; Enterotoxemia; Vacinas; C perfringens; Clostridiose Vacinas
• Link de acesso: http://hdl.handle.net/1843/BUOS-8PQKNM

Seven commercial vaccines against clostridiosis. which had in their composition type C and/or D Closmdzum pemingens toxoids. have been evaluated for sterility, residual toxicity and potency. Six vaccines were produced in Brazil and one was imported, a bivalent toxoid standard of C. perjiingens type C and D has been used as a test control. All tested vaccines were sterile when cultivated in bacteria and fungi growth media. and were innocuous when administrated to mice via intraperitoneal route. ln relation to the potency, evaluated by mice semm neutralization tests using immunized rabbits sera pooled the imported vaccine and the standard toxoid have presented higher serum antibodies titres than the required minimum level of 10 and 5 IU/mL for beta and epsilon toxins produced from type C and D C. peyiirigens, respectively. The brazilian made vaccines were ineffective in stimulating serum antibodies levels specetie to beta and epilon toxins, according to the recommended test level for quality control. Four out of six brazilian made toxoids were also tested in goats but did not presefnt detectable antitoxins levels within the test level of L+ or L+/W used for beta and epsilon toxins. respectively. The toxins utilized have been produced cultivating C. pemingens within in dialisys membrane.