Cinética da infecção experimental e desenvolvimento de PCR multiplex para o diagnóstico de infecções por Brucella ovis, Actinobacillus seminis e Histophilus somni em carneiros

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Valeria Spyridion Moustacas
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Minas Gerais
UFMG
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
PCR
Link de acesso: http://hdl.handle.net/1843/SMOC-9KPQGA
Resumo: Infectious epididymitis is considered a major cause of economic losses for the sheep industry worldwide. The most common causative agents include Brucella ovis, Actinobacillus seminis and Histophilus somni. This study aimed to investigate changes associated with experimental infections with A. seminis and H. somni in rams, and to develop a direct method for differential diagnosis for these three infections. Experimental infections with A. seminis and H. somni were carried out in rams with 18 to 24 months of age. Rams were weekly examined and biological samples were collected for diagnostic purposes. A. seminis and H. somni werecapable of causing infection, colonizing several organs of the genitourinary tract, being indistinguishable only by clinical exam, necropsy or histopathology. Nevertheless, the clinical exam proved to be indispensable as a screening method. Conversely, the importance of laboratorial diagnostic techniques for direct confirmation of the etiologic agent was demonstrated in this study. Therefore, the species-specific multiplex PCR assay developed in this study can be successfully used for the diagnosis of the three most common causes of ovine infectious epididymitis. Furthermore, this technique is a practical alternative to bacteriology. Urine and preputial wash can be used as alternatives to semen samples for DNA extraction and application of the multiplex PCR described in this study.